Lyu Yinghai, Zhang Tingting, Dou Baojuan, Li Guijiang, Ma Chengxin, Li Yangyang
Department of Bioengineering, College of Chemical and Environmental Engineering, Shandong University of Science and Technology Qingdao 266590 PR China
College of Chemical and Environmental Engineering, Shandong University of Science and Technology Qingdao 266590 PR China.
RSC Adv. 2018 Nov 19;8(68):38787-38791. doi: 10.1039/c8ra06693k. eCollection 2018 Nov 16.
The process of using biodesulfurization (BDS) to remove sulfur compounds in petroleum has limitations such as low efficiency and low mass transfer. Therefore, it is important to study the combined effects of biosurfactant and the strain on BDS. A thermophilic desulfurization strain, sp. Lv13, was isolated from the oilfield and used to produce biosurfactant (BS). The strain was identified as , a moderate thermophilic bacterium. Its BS was identified as lipopeptide using thin-layer chromatography (TLC), gas chromatography-mass spectrometry (GC-MS) and Fourier transform infrared spectroscopy (FT-IR). The emulsification efficiency after 24 h ( ) and critical micelle concentration (CMC) were determined to be 46.93% and 30 mg L, respectively. The combined effects of biosurfactant and the strain on BDS was confirmed using the Gibbs assay, GC-MS and BaCl test. Results showed that the yield of 2-hydroxybiphenyl (2-HBP) from dibenzothiophene significantly increased after the addition of lipopeptide into the reaction system. This could be illustrated by the stabilization of emulsion, lower CMC value, higher mass transfer rate with the addition of lipopeptide, and the enhancement in the capacity of BDS as well as the catalytic ability of the microbial cell.
利用生物脱硫（BDS）去除石油中硫化合物的过程存在效率低和传质低等局限性。因此，研究生物表面活性剂和菌株对生物脱硫的联合作用具有重要意义。从油田分离出嗜热脱硫菌株Lv13，并用于生产生物表面活性剂（BS）。该菌株被鉴定为嗜热栖热放线菌，是一种嗜热细菌。使用薄层色谱法（TLC）、气相色谱 - 质谱联用仪（GC - MS）和傅里叶变换红外光谱仪（FT - IR）将其生物表面活性剂鉴定为脂肽。测定24小时后的乳化效率（ ）和临界胶束浓度（CMC）分别为46.93%和30 mg/L。使用吉布斯试验、GC - MS和氯化钡试验证实了生物表面活性剂和菌株对生物脱硫的联合作用。结果表明，向反应体系中添加脂肽后，二苯并噻吩生成2 - 羟基联苯（2 - HBP）的产率显著提高。这可以通过乳液的稳定性、较低的CMC值、添加脂肽后较高的传质速率以及生物脱硫能力和微生物细胞催化能力的增强来解释。
