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油相中存在的茶多酚棕榈酸酯对肌原纤维蛋白水包油乳液稳定性的影响

Effects of Tea Polyphenol Palmitate Existing in the Oil Phase on the Stability of Myofibrillar Protein O/W Emulsion.

作者信息

Li Jianchao, Yang Zongyun, Li Zhen, Wu Ling, Shen Juan, Wang Jinhua, Wang Peng

机构信息

Jiangsu Lihua Animal Husbandry Co., Ltd., Changzhou 213000, China.

Key Laboratory of Meat Processing and Quality Control, MOE, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.

出版信息

Foods. 2022 May 2;11(9):1326. doi: 10.3390/foods11091326.

Abstract

This study aimed to explore the effect of adding different concentrations (0, 0.01%, 0.03%, and 0.05% (w/w)) of tea polyphenol palmitate (TPP) in the oil phase on the emulsifying properties of 5 and 10 mg/mL myofibrillar protein (MP). Particle size results revealed that the flocculation of droplets increased as TPP concentration increased and that droplets in 5 mg/mL MP emulsions (25−34 μm) were larger than in 10 mg/mL MP emulsions (14−22 μm). The emulsifying activity index of 5 mg/mL MP emulsions decreased with increasing TPP concentration. The micrographs showed that the droplets of MP emulsions exhibited extensive flocculation at TPP concentrations >0.03%. Compared with 5 mg/mL MP emulsions, 10 mg/mL MP emulsions showed better physical stability and reduced flocculation degree, which coincided with lower delta backscattering intensity (ΔBS) and Turbiscan stability index values. The flow properties of emulsions can be successfully depicted by Ostwald−de Waele models (R2 > 0.99). The concentrations of TPP and protein affect the K values of emulsions (p < 0.05). Altogether, increased protein concentration in the continuous phase could improve emulsion stability by increasing viscosity, offsetting the adverse effects of TPP to a certain extent. This study is expected to promote the rational application of TPP in protein emulsion products of high quality and acceptability.

摘要

本研究旨在探讨在油相中添加不同浓度(0、0.01%、0.03%和0.05%(w/w))的茶多酚棕榈酸酯(TPP)对5和10 mg/mL肌原纤维蛋白(MP)乳化性能的影响。粒径结果显示,随着TPP浓度的增加,液滴的絮凝作用增强,且5 mg/mL MP乳液中的液滴(25−34μm)比10 mg/mL MP乳液中的液滴(14−22μm)更大。5 mg/mL MP乳液的乳化活性指数随TPP浓度的增加而降低。显微照片显示,当TPP浓度>0.03%时,MP乳液的液滴出现广泛絮凝。与5 mg/mL MP乳液相比,10 mg/mL MP乳液表现出更好的物理稳定性和更低的絮凝程度,这与更低的背散射强度增量(ΔBS)和Turbiscan稳定性指数值一致。乳液的流动特性可以通过奥斯特瓦尔德−德瓦勒模型成功描述(R2>0.99)。TPP和蛋白质的浓度会影响乳液的K值(p<0.05)。总之,连续相中蛋白质浓度的增加可通过增加粘度来提高乳液稳定性,在一定程度上抵消TPP的不利影响。本研究有望促进TPP在高品质和可接受性的蛋白质乳液产品中的合理应用。

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