Gnutikov Alexander A, Nosov Nikolai N, Loskutov Igor G, Blinova Elena V, Shneyer Viktoria S, Probatova Nina S, Rodionov Alexander V
Department of Genetic Resources of Oat, Barley, Rye, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, Russia.
Laboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, Russia.
Plants (Basel). 2022 Apr 19;11(9):1103. doi: 10.3390/plants11091103.
We used next-generation sequencing analysis of the 3′-part of 18S rDNA, ITS1, and a 5′-part of the 5.8S rDNA region to understand genetic variation among seven diploid A-genome Avena species. We used 4−49 accessions per species that represented the As genome (A. atlantica, A. hirtula, and wiestii), Ac genome (A. canariensis), Ad genome (A. damascena), Al genome (A. longiglumis), and Ap genome (A. prostrata). We also took into our analysis one C-genome species, A. clauda, which previously was found to be related to A-genome species. The sequences of 169 accessions revealed 156 haplotypes of which seven haplotypes were shared by two to five species. We found 16 ribotypes that consisted of a unique sequence with a characteristic pattern of single nucleotide polymorphisms and deletions. The number of ribotypes per species varied from one in A. longiglumis to four in A. wiestii. Although most ribotypes were species-specific, we found two ribotypes shared by three species (one for A. damascena, A. hirtula, and A. wiestii, and the second for A. longiglumis, A. atlantica, and A. wiestii), and a third ribotype shared between A. atlantica and A. wiestii. A characteristic feature of the A. clauda ribotype, a diploid C-genome species, is that two different families of ribotypes have been found in this species. Some of these ribotypes are characteristic of Cc-genome species, whereas others are closely related to As-genome ribotypes. This means that A. clauda can be a hybrid between As- and C-genome oats.
我们使用下一代测序分析法对18S rDNA的3′端、ITS1以及5.8S rDNA区域的5′端进行分析，以了解7个二倍体A基因组燕麦物种之间的遗传变异。我们每个物种使用了4 - 49份代表As基因组（大西洋燕麦、硬毛燕麦和维氏燕麦）、Ac基因组（加那利燕麦）、Ad基因组（大马士革燕麦）、Al基因组（长颖燕麦）和Ap基因组（平卧燕麦）的材料。我们还将一个C基因组物种克劳德燕麦纳入分析，该物种先前被发现与A基因组物种有关。169份材料的序列揭示了156种单倍型，其中7种单倍型为2至5个物种所共有。我们发现了16种核糖型，它们由具有单核苷酸多态性和缺失特征模式的独特序列组成。每个物种的核糖型数量从长颖燕麦的1种到维氏燕麦的4种不等。虽然大多数核糖型是物种特异性的，但我们发现有两种核糖型为3个物种所共有（一种为大马士革燕麦、硬毛燕麦和维氏燕麦所共有，另一种为长颖燕麦、大西洋燕麦和维氏燕麦所共有），还有第三种核糖型为大西洋燕麦和维氏燕麦所共有。克劳德燕麦（一种二倍体C基因组物种）核糖型的一个特征是，在该物种中发现了两个不同的核糖型家族。其中一些核糖型是Cc基因组物种所特有的，而另一些则与As基因组核糖型密切相关。这意味着克劳德燕麦可能是As基因组和C基因组燕麦的杂交种。
