Department of Polymeric Materials and Engineering, School of Materials and Energy, Guangdong University of Technology, Guangzhou 510006, China.
Guangdong Provincial Key Laboratory of Functional Soft Condensed Matter, Guangzhou 510006, China.
ACS Macro Lett. 2022 Jun 21;11(6):716-722. doi: 10.1021/acsmacrolett.2c00228. Epub 2022 May 10.
We report a strategy toward surface-functional polymeric microspheres using a wavelength orthogonality technique that employs photoinitiated reversible addition-fragmentation chain transfer (RAFT) dispersion polymerization and the subsequent photografting under different wavelengths of light. Initial screening of reaction conditions indicated photoreactive polymeric microsphere with uniform sizes could be prepared by using photoinitiator-functionalized macro-RAFT agents under purple light irradiation. Photoreactive polymeric microspheres allowed photografting polymerizations under UV light irradiation, and we further demonstrated the broad scope of this method by photografting acrylamide, acrylic, and methacrylic monomers. Finally, carboxyl-functionalized polymeric microspheres with an exceptional high number of carboxyl groups were successfully prepared by this technique, which permitted extensive surface bioconjugation of model proteins (e.g., streptavidin). This method should expand the capabilities of RAFT dispersion polymerization to afford diverse surface-functional polymeric microspheres for some specific applications.
我们报告了一种使用波长正交技术的表面功能化聚合物微球的策略，该技术采用光引发可逆加成-断裂链转移（RAFT）分散聚合，然后在不同波长的光下进行光接枝。反应条件的初步筛选表明，在紫光照射下，使用光功能化的大分子 RAFT 试剂可以制备具有均匀粒径的光反应性聚合物微球。光反应性聚合物微球允许在紫外光照射下进行光接枝聚合，我们进一步通过光接枝丙烯酰胺、丙烯酸和甲基丙烯酸单体证明了该方法的广泛适用性。最后，通过该技术成功制备了具有异常高数量羧基的羧基功能化聚合物微球，这允许模型蛋白质（例如链霉亲和素）的广泛表面生物偶联。该方法应扩展 RAFT 分散聚合的能力，以提供用于某些特定应用的各种表面功能化聚合物微球。
