蜜蜂蜂王和工蜂品级发育过程中RNA加工的程度和复杂性。

Extent and complexity of RNA processing in honey bee queen and worker caste development.

作者信息

He Xu Jiang, Barron Andrew B, Yang Liu, Chen Hu, He Yu Zhu, Zhang Li Zhen, Huang Qiang, Wang Zi Long, Wu Xiao Bo, Yan Wei Yu, Zeng Zhi Jiang

机构信息

Honeybee Research Institute, Jiangxi Agricultural University, Nanchang, Jiangxi 330045, P. R. of China.

Jiangxi Province Honeybee Biology and Beekeeping Nanchang, Jiangxi 330045, P. R. of China.

出版信息

iScience. 2022 Apr 25;25(5):104301. doi: 10.1016/j.isci.2022.104301. eCollection 2022 May 20.

DOI:10.1016/j.isci.2022.104301

PMID:35573188

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9097701/

Abstract

The distinct honeybee () worker and queen castes have become a model for the study of genomic mechanisms of phenotypic plasticity. Here we performed a nanopore-based direct RNA sequencing with exceptionally long reads to compare the mRNA transcripts between queen and workers at three points during their larval development. We found thousands of significantly differentially expressed transcript isoforms (DEIs) between queen and worker larvae. These DEIs were formatted by a flexible splicing system. We showed that poly(A) tails participated in this caste differentiation by negatively regulating the expression of DEIs. Hundreds of isoforms uniquely expressed in either queens or workers during their larval development, and isoforms were expressed at different points in queen and worker larval development demonstrating a dynamic relationship between isoform expression and developmental mechanisms. These findings show the full complexity of RNA processing and transcript expression in honey bee phenotypic plasticity.

摘要

独特的蜜蜂工蜂和蜂王等级已成为研究表型可塑性基因组机制的模型。在这里，我们进行了基于纳米孔的直接RNA测序，获得了超长读长，以比较蜂王和工蜂幼虫发育三个阶段的mRNA转录本。我们发现蜂王和工蜂幼虫之间有成千上万个显著差异表达的转录本异构体（DEIs）。这些DEIs由一个灵活的剪接系统形成。我们表明，聚腺苷酸尾通过负调控DEIs的表达参与了这种等级分化。数百种异构体在蜂王或工蜂幼虫发育过程中独特表达，并且异构体在蜂王和工蜂幼虫发育的不同阶段表达，表明异构体表达与发育机制之间存在动态关系。这些发现揭示了蜜蜂表型可塑性中RNA加工和转录本表达的全部复杂性。