Sánchez Antonio, Contreras Antonio, Sánchez-Corral María L, Martínez-Nista Carmen, Collado Soledad, Sáez José L, Minguez Olga, de la Fe Christian
Ruminant Health Research Group, Department of Animal Health, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, 30100 Murcia, Spain.
Servicio de Sanidad Animal de la Dirección General de Producción Agropecuaria de la Consejería de Agricultura, Ganadería y Desarrollo Rural de la Junta de Castilla y León, León Spain.
J Vet Res. 2022 Mar 10;66(1):95-101. doi: 10.2478/jvetres-2022-0010. eCollection 2022 Mar.
Contagious agalactia (CA) is a disease affecting small ruminants with worldwide distribution and caused by several mycoplasmas, especially . The main option for systematic diagnosis under monitoring control programmes is the enzyme-linked immunosorbent assay (ELISA) test.
This study was designed to appraise the performance of two commercial indirect ELISA tests using p48 protein and one using total protein, for antibody detection in small ruminants after natural infection with different strains. We carried out the test evaluation using sera of confirmed -positive goats with clinical signs. In addition, test agreement was assessed by kappa between the three commercial ELISA tests.
All three ELISA tests showed high validity scores (Youden's J: 72.9-84%). The sensitivity values for the P48 protein-based tests were 76.9% and 84.6%, and was 79% for the total protein-based test. The specificity of all tests was 100%. In addition, between the total protein-based ELISA test and the other two ELISA tests based on the P48 protein, the agreement was substantial (kappa: 0.762-0.763) and the agreement between the latter two tests was almost perfect (kappa: 0.93).
The validity parameters for all tests allowed their application for diagnostic purposes in lactating goats excreting in milk and presenting clinical signs. The agreements show that any of these ELISA tests could be equally well used for diagnosis in programmes against CA.
传染性无乳症（CA）是一种影响小反刍动物的疾病，在全球范围内均有分布，由多种支原体引起，尤其是[具体支原体未给出]。在监测控制项目下进行系统诊断的主要方法是酶联免疫吸附测定（ELISA）试验。
本研究旨在评估两种使用p48蛋白的商业间接ELISA试验和一种使用总蛋白的ELISA试验在不同[具体支原体未给出]菌株自然感染后的小反刍动物中检测抗体的性能。我们使用具有临床症状的确诊阳性山羊血清进行试验评估。此外，通过kappa值评估三种商业ELISA试验之间的一致性。
所有三种ELISA试验均显示出较高的有效性评分（约登指数J：72.9 - 84%）。基于p48蛋白的试验的敏感性值分别为76.9%和84.6%，基于总蛋白的试验的敏感性值为79%。所有试验的特异性均为100%。此外，基于总蛋白的ELISA试验与另外两种基于p48蛋白的ELISA试验之间的一致性较高（kappa值：0.762 - 0.763），而后两种试验之间的一致性几乎完美（kappa值：0.93）。
所有试验的有效性参数使其可应用于诊断泌乳山羊，这些山羊在乳汁中排出[具体支原体未给出]并表现出临床症状。一致性表明，这些ELISA试验中的任何一种都可同样有效地用于CA防治项目中的诊断。
