Kashem Mohammad Abul, Li Lin, Yuan Xin-Yong, Plummer Francis A, Luo Ma
Department of Medical Microbiology and Infectious Diseases, University of Manitoba, Winnipeg, MB, Canada.
JC Wilt Infectious Diseases Research Center, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada.
J Inflamm Res. 2022 May 12;15:2925-2937. doi: 10.2147/JIR.S357866. eCollection 2022.
TILRR is a modulator of genes in the NF-κB inflammation pathway. It regulates inflammation-responsive genes, the secretion of inflammatory mediators, and the migration of immune cells. Because inflammation drives the pathogenesis of many infectious and inflammatory diseases, it is important to know the expression of TILRR protein in tissues and cells. This study examined TILRR protein expression in healthy adult human and macaques' tissues and PBMCs (peripheral blood mononuclear cells).
Tissues (trachea, lungs, stomach, small intestine [ileum], cecum, colon, rectum, vagina, cervix, uterus, and penis) and PBMCs from humans and macaques were lysed in RIPA (radioimmunoprecipitation assay) lysis buffer. The TILRR protein was examined by fluorescent Western blot analysis. The relative fluorescence units (rfu) of TILRR protein expression were quantified by Image Studio software (LI-COR). The results showed that adult healthy female (n=1) rectal and cervicovaginal tissues expressed a higher level of TILRR protein than the other tissues (trachea, lungs, stomach, small intestine [ileum], cecum, colon, uterus, and penis) examined. Like humans, the lungs, colon, and rectal tissues of healthy adult female cynomolgus monkeys () (n=2) expressed the TILRR protein. In addition, PBMCs of healthy adult women (n=4), adult female cynomolgus monkeys () (n=4), and adult male and female rhesus monkeys () (n=4) showed a similar expression level of TILRR protein (= 0.2858). TILRR protein was not detected in most of the human cell lines examined, except in Jurkat cells.
Our study for the first time showed that TILRR protein is expressed in healthy adult human and monkey tissues and PBMCs. The TILRR protein in these tissues and PBMCs may play a role in the inflammatory response of these tissues and cells in response to infectious pathogens.
TILRR是核因子κB炎症通路中基因的调节剂。它调节炎症反应基因、炎症介质的分泌以及免疫细胞的迁移。由于炎症驱动许多感染性和炎症性疾病的发病机制，了解TILRR蛋白在组织和细胞中的表达情况很重要。本研究检测了健康成年人类和猕猴组织及外周血单核细胞（PBMC）中TILRR蛋白的表达。
将人类和猕猴的组织（气管、肺、胃、小肠[回肠]、盲肠、结肠、直肠、阴道、子宫颈、子宫和阴茎）及PBMC在RIPA（放射免疫沉淀分析）裂解缓冲液中裂解。通过荧光免疫印迹分析检测TILRR蛋白。用Image Studio软件（LI-COR）对TILRR蛋白表达的相对荧光单位（rfu）进行定量。结果显示，成年健康女性（n = 1）的直肠和宫颈阴道组织中TILRR蛋白表达水平高于其他检测组织（气管、肺、胃、小肠[回肠]、盲肠、结肠、子宫和阴茎）。与人类相似，成年健康雌性食蟹猴（n = 2）的肺、结肠和直肠组织表达TILRR蛋白。此外，成年健康女性（n = 4）、成年雌性食蟹猴（n = 4）以及成年雄性和雌性恒河猴（n = 4）的PBMC中TILRR蛋白表达水平相似（P = 0.2858）。在所检测的大多数人类细胞系中未检测到TILRR蛋白，除了Jurkat细胞。
我们的研究首次表明TILRR蛋白在健康成年人类和猴组织及PBMC中表达。这些组织和PBMC中的TILRR蛋白可能在这些组织和细胞对感染性病原体的炎症反应中发挥作用。
