Burge D M, Holbrook A G, Karran S J
J Pediatr Surg. 1987 Mar;22(3):211-4. doi: 10.1016/s0022-3468(87)80330-5.
A simple reproducible animal model of extrahepatic portal hypertension (EHPHT) has been developed in weanling Wistar rats using a two-stage ligation of the portal vein. This model consistently produces substantial collaterals, both portosystemic (hepatofugal) and portoportal (hepatopetal). Using dynamic hepatic scintigraphy (DHS) with 99mTechnetium sulphurcolloid, hepatopetal collateral flow was measured as the mesenteric fraction (MF) of total hepatic blood flow and compared with measurement of hepatofugal collateral flow (portosystemic shunting) following intraportal injection of radiolabeled microspheres. Strong and significant correlation between the two assessments was found with reduction in MF denoting increased portosystemic shunting (PSS). The technique of DHS has been used successfully in adults to assess compromised portal venous flow and is a simple noninvasive test to aid diagnosis, assessment, and follow-up of children with EHPHT.
利用门静脉两阶段结扎法，在断乳的Wistar大鼠中建立了一种简单可重复的肝外门静脉高压（EHPHT）动物模型。该模型持续产生大量侧支循环，包括门体（离肝性）和门门（向肝性）侧支循环。使用锝-99m硫胶体进行动态肝脏闪烁扫描（DHS），将向肝性侧支血流测量为总肝血流的肠系膜分数（MF），并与门静脉内注射放射性标记微球后离肝性侧支血流（门体分流）的测量结果进行比较。发现这两种评估之间存在强且显著的相关性，MF降低表示门体分流（PSS）增加。DHS技术已成功用于成人评估受损的门静脉血流，并且是一种简单的非侵入性检查，有助于EHPHT患儿的诊断、评估和随访。
