Pollmann Marie, Moore Logan D, Krimmer Elena, D'Alvise Paul, Hasselmann Martin, Perlman Steve J, Ballinger Matthew J, Steidle Johannes L M, Gottlieb Yuval
Department of Chemical Ecology 190t, Institute of Biology, University of Hohenheim, 70599 Stuttgart, Germany.
Department of Biological Sciences, Mississippi State University, Mississippi State, MS 39762, USA.
iScience. 2022 Apr 29;25(5):104335. doi: 10.1016/j.isci.2022.104335. eCollection 2022 May 20.
Cytoplasmic incompatibility (CI) is a form of reproductive manipulation caused by maternally inherited endosymbionts infecting arthropods, like , whereby matings between infected males and uninfected females produce few or no offspring. We report the discovery of a new CI symbiont, a strain of causing CI in the parasitoid wasp . Its extracellular occurrence enabled us to establish CI in uninfected adult insects by transferring -infected hemolymph. We sequenced the CI- genome and did not find any homologues of any of the genes discovered to cause CI in , suggesting independent evolution of CI. Instead, the genome contains other potential CI-causing candidate genes, such as homologues of high-mobility group (HMG) box proteins that are crucial in eukaryotic development but rare in bacterial genomes. 's extracellular nature and broad host range encompassing medically and agriculturally important arthropods make it a promising tool to study CI and its applications.
细胞质不亲和（CI）是一种由母体遗传的共生菌感染节肢动物（如 ）所引起的生殖操纵形式，受感染的雄性与未受感染的雌性交配产生很少或没有后代。我们报告发现了一种新的CI共生菌，是一种在寄生蜂 中引起CI的 菌株。其细胞外存在使我们能够通过转移感染 的血淋巴在未感染的成年昆虫中建立CI。我们对引起CI的 基因组进行了测序，未发现任何在 中发现的导致CI的 基因的同源物，这表明CI是独立进化的。相反，该基因组包含其他潜在的导致CI的候选基因，例如高迁移率族（HMG）盒蛋白的同源物，这些蛋白在真核生物发育中至关重要，但在细菌基因组中很少见。 的细胞外性质以及涵盖医学和农业上重要节肢动物的广泛宿主范围使其成为研究CI及其应用的有前途的工具。
