[Qualitative characteristics of Soviet kits for the radioimmunological determination of cortisol and progesterone in human blood serum].

Radioimmunoassays and kits of reagents based on them CORT-I-3H,STERON-K-125I, STERON-P-3H and STERON-P-125I intended for medical microanalysis, were studied. Their development and serial production were undertaken in the Institute of Bioorganic Chemistry, AS BSSR. The data obtained characterized the quality of the radioimmunoassays under study, long-term stability and reliability of the kits of reagents. A high specificity of antisera and correlation between the results of measurements and a degree of native blood serum dilution as well as the accuracy of the results obtained and sensitivity of the assays as a whole were demonstrated. The above properties of the radioimmunoassays made it possible to develop serial kits of reagents for determination of cortisol and progesterone concentration in the human blood serum, employed effectively in medical diagnosis of endocrine and other diseases. A long-term control over the reproducibility of parameters of calibration charts and the results of determination of the level of cortisol and progesterone in the pool blood serum of women showed a high reliability of the kits of reagents of different production lots within the time of their application observing instructions for use.