Veterinary Teaching Hospital, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
Laboratory of Advanced Veterinary Medicine, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan.
J Vet Med Sci. 2022 Jul 10;84(7):954-959. doi: 10.1292/jvms.22-0131. Epub 2022 May 27.
The urinary corticoid to creatinine ratio (UCCR) is one of the most commonly used screening tests for canine hypercortisolism (HC). In this study, a reference interval was established for UCCR using IMMULITE 2000 XPi, the latest chemiluminescence enzyme immunoassay. The diagnostic performance of this method for UCCR in canine HC was also evaluated. The median UCCR was 1.06 × 10 (range: 0.28-2.49) for 58 healthy dogs, and an upper reference limit of 1.98 × 10 (90% confidence interval: 1.76-2.15) was determined. The median UCCR in the 12 dogs with HC (7.38 × 10, range 1.86-29.98) was significantly higher than that in the 16 dogs with mimic-HC (1.59 × 10, range 0.47-3.42, P<0.001). The area under the curve for UCCR to differentiate HC dogs from mimic-HC dogs was 0.971, with a sensitivity of 91.7% and specificity of 100% when the cut-off value was set at 3.77 × 10. The UCCR of 16 paired urine samples collected at home and in hospital showed that the UCCR of samples collected in the hospital was significantly higher than that of samples collected at home (mean difference 3.30 × 10, 95% confidence interval: 0.70-5.90, P=0.001). In summary, we established the upper reference limit for UCCR using IMMULITE 2000 XPi in dogs and confirmed that UCCR is a useful diagnostic test for HC in dogs if urine samples are collected at home.
尿皮质醇与肌酐比值（UCCR）是犬库欣病（HC）最常用的筛查试验之一。本研究使用最新的化学发光酶免疫分析法 IMMULITE 2000 XPi 建立了 UCCR 的参考区间，并评估了该方法对犬 HC 的诊断性能。58 只健康犬的 UCCR 中位数为 1.06×10（范围：0.28-2.49），确定了 1.98×10（90%置信区间：1.76-2.15）的上参考限。12 只 HC 犬的 UCCR 中位数为 7.38×10（范围 1.86-29.98），明显高于 16 只假性 HC 犬的 UCCR 中位数 1.59×10（范围 0.47-3.42，P<0.001）。UCCR 区分 HC 犬和假性 HC 犬的曲线下面积为 0.971，当截断值设定为 3.77×10 时，敏感性为 91.7%，特异性为 100%。在医院和家中采集的 16 对尿液样本的 UCCR 显示，医院采集的样本 UCCR 明显高于家中采集的样本（平均差值 3.30×10，95%置信区间：0.70-5.90，P=0.001）。总之，我们使用 IMMULITE 2000 XPi 建立了犬 UCCR 的上参考限，并证实如果在家中采集尿液样本，UCCR 是犬 HC 的一种有用的诊断试验。
