Chao Wun S, Li Xuehui, Horvath David P, Anderson James V
Edward T. Schafer Agricultural Research Center, Sunflower and Plant Biology Research Unit USDA-Agricultural Research Service Fargo North Dakota USA.
Department of Plant Sciences North Dakota State University Fargo North Dakota USA.
Plant Direct. 2022 May 25;6(5):e405. doi: 10.1002/pld3.405. eCollection 2022 May.
Winter biotypes of rapeseed ( L.) require a vernalization treatment to enter the reproductive phase and generally produce greater yields than spring rapeseed. To find genetic loci associated with freezing tolerance in rapeseed, we first performed genotyping-by-sequencing (GBS) on a diversity panel consisting of 222 rapeseed accessions originating primarily from Europe, which identified 69,554 high-quality single-nucleotide polymorphisms (SNPs). Model-based cluster analysis suggested that there were eight subgroups. The diversity panel was then phenotyped for freezing survival (visual damage and Fv/Fo and Fv/Fm) after 2 months of cold acclimation (5°C) and a freezing treatment (-15°C for 4 h). The genotypic and phenotypic data for each accession in the rapeseed diversity panel was then used to conduct a genome-wide association study (GWAS). GWAS results showed that 14 significant markers were mapped to seven chromosomes for the phenotypes scored. Twenty-four candidate genes located within the mapped loci were identified as previously associated with lipid, photosynthesis, flowering, ubiquitination, and cytochrome P450 in rapeseed or other plant species.
油菜（L.）的冬性生态型需要经过春化处理才能进入生殖阶段，并且通常比春油菜产量更高。为了找到与油菜耐冻性相关的基因位点，我们首先对一个由222份主要源自欧洲的油菜种质组成的多样性群体进行了简化基因组测序（GBS），共鉴定出69554个高质量单核苷酸多态性（SNP）。基于模型的聚类分析表明存在八个亚组。然后，在经过2个月的低温驯化（5°C）和一次冷冻处理（-15°C处理4小时）后，对该多样性群体进行了冻害存活率（视觉损伤以及Fv/Fo和Fv/Fm）的表型分析。随后，利用油菜多样性群体中每个种质的基因型和表型数据进行全基因组关联研究（GWAS）。GWAS结果显示，针对所评分的表型，有14个显著标记被定位到七条染色体上。位于所定位位点内的24个候选基因被鉴定为先前在油菜或其他植物物种中与脂质、光合作用、开花、泛素化和细胞色素P450相关的基因。
