利用模板片段连接鉴定蛋白质-蛋白质相互作用稳定剂抑制性肽-片段杂交体。
Towards identification of protein-protein interaction stabilizers inhibitory peptide-fragment hybrids using templated fragment ligation.
作者信息
Srdanović Sonja, Hegedüs Zsofia, Warriner Stuart L, Wilson Andrew J
机构信息
Astbury Centre for Structural Molecular Biology, University of Leeds, Woodhouse Lane Leeds LS2 9JT UK
School of Chemistry, University of Leeds, Woodhouse Lane Leeds LS2 9JT UK.
出版信息
RSC Chem Biol. 2022 Apr 1;3(5):546-550. doi: 10.1039/d2cb00025c. eCollection 2022 May 11.
Abstract
Using the DMX/14-3-3 interaction, acylhydrazone-based ligand-directed fragment ligation was used to identify protein-protein interaction (PPI) inhibitory peptide-fragment hybrids. Separation of the peptide-fragment hybrids into the components yielded fragments that stabilized the DMX/14-3-3 interaction.
摘要
利用DMX/14-3-3相互作用，基于酰腙的配体导向片段连接被用于鉴定蛋白质-蛋白质相互作用（PPI）抑制性肽-片段杂合物。将肽-片段杂合物分离成各个组分，得到了能稳定DMX/14-3-3相互作用的片段。
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