Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Indian J Med Microbiol. 2022 Jul-Sep;40(3):389-393. doi: 10.1016/j.ijmmb.2022.05.002. Epub 2022 May 31.
Free-living amoebae (FLA) exist in various environmental sources and are the causative agents of many serious human diseases. We surveyed various natural and artificial water bodies in areas of north India to detect the presence of FLA species.
Surface water samples were collected from 29 water bodies and subjected to microscopy, non-nutrient agar (NNA) culture, and various polymerase chain reaction (PCR) assays for the detection of FLA.
FLA were found in 62.1% (18/29) of the natural and artificial water bodies by NNA culture. Based on PCR based detection, Acanthamoeba spp. was identified in 41.4% (12/29), Vannella spp./Vahlkampfia ovis in 17.2% (5/29) and Naegleria spp. in 20.7% (6/29) of the samples from different locations. PCR-sequencing of Naegleria positive samples identified them as Naegleria australiensis and Naegleria species.
The present study reports widespread occurrence of medically important FLA species in various natural and artificial water bodies, and highlights the need for regular molecular surveillance of FLA in the environment for better control and prevention of the diseases caused by FLA.
自由生活的阿米巴（FLA）存在于各种环境源中，是许多严重人类疾病的病原体。我们调查了印度北部地区的各种自然和人工水体，以检测 FLA 物种的存在。
从 29 个水体中采集地表水样本，并进行显微镜检查、非营养琼脂（NNA）培养以及各种聚合酶链反应（PCR）检测，以检测 FLA。
NNA 培养发现 62.1%（18/29）的自然和人工水体中存在 FLA。基于基于 PCR 的检测，在来自不同地点的 41.4%（12/29）的样本中鉴定出棘阿米巴属/ Vahlkampfia ovis 在 17.2%（5/29）的样本中鉴定出 Naegleria spp. 在 20.7%（6/29）的样本中。Naegleria 阳性样本的 PCR 测序将其鉴定为 Naegleria australiensis 和 Naegleria 种。
本研究报告了各种自然和人工水体中存在广泛分布的具有医学重要性的 FLA 物种，并强调需要定期对环境中的 FLA 进行分子监测，以更好地控制和预防由 FLA 引起的疾病。
Zotero 插件