利用 FLP/FRT 系统筛选果蝇翅 Notch 信号通路调控因子

Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing.

机构信息

Department of Entomology and MOA Key Lab of Pest Monitoring and Green Management, College of Plant Protection, China Agricultural University, Beijing, China.

出版信息

Methods Mol Biol. 2022;2472:39-48. doi: 10.1007/978-1-0716-2201-8_4.

DOI:10.1007/978-1-0716-2201-8_4

PMID:35674890

Abstract

Mutations of genes encoding key components of the Notch signaling pathways often result in lethality at early developmental stages, making it difficult to decipher how they regulate the formation of specific cell types or organs. Mosaic analysis using the FLP/FRT system allows investigating the roles of essential genes during wing development in Drosophila melanogaster. This chapter describes the practical methods to isolate Notch signaling regulators by somatic mosaic screen. The fly stocks, cross schemes, and screen parameters are summarized. We also explain how to validate the roles of potential Notch signaling regulators.

摘要

基因突变编码 Notch 信号通路关键成分的基因通常会导致早期发育阶段的致死，这使得很难解释它们如何调节特定细胞类型或器官的形成。使用 FLP/FRT 系统的嵌合体分析可以研究果蝇翅膀发育过程中必需基因的作用。本章描述了通过体细胞嵌合体筛选分离 Notch 信号调节剂的实用方法。总结了蝇株、杂交方案和筛选参数。我们还解释了如何验证潜在 Notch 信号调节剂的作用。