效应器 Vγ9Vδ2 T 细胞的生成及其对磷酸抗原负载细胞反应的评估。
Generation of effector Vγ9Vδ2 T cells and evaluation of their response to phosphoantigen-loaded cells.
机构信息
Department of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269, USA.
Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269, USA.
出版信息
STAR Protoc. 2022 Jun 4;3(2):101422. doi: 10.1016/j.xpro.2022.101422. eCollection 2022 Jun 17.
Vγ9Vδ2 T cells are non-canonical T cells that use their T cell receptor to detect phosphoantigens bound to the internal domain of the HMBPP receptor (butyrophilin 3/2A1 complex). This protocol describes the expansion and purification of human effector Vγ9Vδ2 T cells from human buffy coat and describes how to assess their activation by antigen-containing target cells. While specifically focused on cytokine production, this protocol can be readily adapted to evaluate other effector functions of activated Vγ9Vδ2 T cells. For complete details on the use and execution of this protocol, please refer to Hsiao et al. (2022) and Hsiao and Wiemer (2018).
Vγ9Vδ2 T 细胞是一种非典型的 T 细胞，它使用其 T 细胞受体来检测与 HMBPP 受体（丁酰膦蛋白 3/2A1 复合物）内部结构域结合的磷酸抗原。本方案描述了从人血白细胞中扩增和纯化人类效应 Vγ9Vδ2 T 细胞的方法，并描述了如何通过含有抗原的靶细胞来评估它们的激活情况。虽然本方案特别侧重于细胞因子的产生，但可以很容易地将其改编用于评估活化的 Vγ9Vδ2 T 细胞的其他效应功能。有关此方案的使用和执行的完整详细信息，请参见 Hsiao 等人（2022 年）和 Hsiao 和 Wiemer（2018 年）。
Zotero 插件