用 Treg、G-MDSC 和 IL-2 治疗 EAE 小鼠：多发性硬化症细胞治疗的新见解。

Treatment of EAE mice with Treg, G-MDSC and IL-2: a new insight into cell therapy for multiple sclerosis.

机构信息

Medical Cellular & Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran.

Immunogenetics Research Center, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

Immunotherapy. 2022 Jul;14(10):789-798. doi: 10.2217/imt-2021-0045. Epub 2022 Jun 8.

DOI:10.2217/imt-2021-0045

PMID:35678041

Abstract

This study investigates the therapeutic and protective effects of Tregs, myeloid-derived suppressor cells (MDSCs) and IL-2 on multiple sclerosis (MS) disease model. C57BL/6 mice were immunized to develop an experimental autoimmune encephalomyelitis (EAE) model. We then investigated effects of pre- and post-treatment EAE mice with Tregs, MDSCs and IL-2 on inflammation and demyelination in brain tissue, and on the number of Treg, granulocytic-MDSC and Th-17 cells in spleen. Pre- and post-treatment of EAE mice by Tregs, MDSCs and IL-2 resulted in no weight change, reduced Th-17 cells and suppression of pathological properties. Pre- and post-treatment of immunized mice by Tregs, MDSCs and IL-2 prevent EAE induction.

摘要

本研究探讨了调节性 T 细胞（Tregs）、髓系来源抑制细胞（MDSCs）和白细胞介素-2（IL-2）对多发性硬化症（MS）疾病模型的治疗和保护作用。通过免疫 C57BL/6 小鼠来建立实验性自身免疫性脑脊髓炎（EAE）模型。然后，我们研究了 Tregs、MDSCs 和 IL-2 对脑内炎症和脱髓鞘的影响，以及对脾内 Treg、粒细胞-MDSC 和 Th17 细胞数量的影响。在 EAE 小鼠中进行 Tregs、MDSCs 和 IL-2 的预处理和后处理，没有导致体重变化，减少了 Th17 细胞并抑制了病理特性。通过 Tregs、MDSCs 和 IL-2 对免疫小鼠进行预处理和后处理可以预防 EAE 的诱导。