Matsumura T, Sugimoto T, Sawada T, Amagai T, Negoro S, Kemshead J T
Cancer Res. 1987 Jun 1;47(11):2924-30.
Neuroblastoma (NB) arises from primitive sympathetic neuroblasts in the adrenal gland or the sympathetic ganglion. NB in situ, sometimes observed in the adrenal glands of autopsied infants, is considered to be a premalignant lesion that may develop into NB. Little is understood about the morphological and biochemical changes that accompany this malignant progression. In this study, a unique monoclonal antibody, KP-NAC8, raised against a human NB cell line is described. This binds to NB cells but not to fetal neuroblasts. The antibody recognizes a Mr 200,000 surface protein on NB cells. KP-NAC8 binds to 15 of 17 human NB cell lines and all 26 fresh NB samples either from tumor tissues or from marrow aspirates involved with tumor. The antibody was found to cross-react with some other tumor cell lines, namely, Ewing's sarcoma (1 of 2), melanoma (1 of 4), lung cancer (3 of 3), and leukemia (2 of 14) cell lines. However, KP-NAC8 did not bind to any rhabdomyosarcoma (0 of 4), Wilms' tumor (0 of 4), retinoblastoma (0 of 2), glioma (0 of 4), and gastric cancer (0 of 2) cell lines examined. Among fetal tissues, KP-NAC8 did not react with normal neuroblasts in the adrenal glands of 5 fetuses. In a further study, the membrane phenotype of fetal adrenal neuroblasts was analyzed by a panel of 12 monoclonal antibodies including KP-NAC8. A comparison of the binding of the same panel of antibodies to fresh NB revealed that antibodies UJ13A, UJ127:11, PI153/3, anti-Thy-1, A2B5, BA-1, BA-2, HSAN1.2, and Leu-7 bound to both fetal adrenal neuroblasts and NB cells. Monoclonal antibodies OKIa-1 and J5 did not bind to either tissues. The only antibody that could distinguish fetal adrenal neuroblasts from NB cells was KP-NAC8. KP-NAC8 may, therefore, define a differentiation-related antigen that may prove helpful in understanding the biological nature of NB and NB in situ.
神经母细胞瘤（NB）起源于肾上腺或交感神经节中的原始交感神经母细胞。原位NB有时在尸检婴儿的肾上腺中观察到，被认为是一种可能发展为NB的癌前病变。对于伴随这种恶性进展的形态学和生化变化了解甚少。在本研究中，描述了一种针对人NB细胞系产生的独特单克隆抗体KP-NAC8。它与NB细胞结合，但不与胎儿神经母细胞结合。该抗体识别NB细胞上一种分子量为200,000的表面蛋白。KP-NAC8与17个人NB细胞系中的15个以及所有26个来自肿瘤组织或与肿瘤相关的骨髓穿刺液的新鲜NB样本结合。发现该抗体与其他一些肿瘤细胞系发生交叉反应，即尤因肉瘤（2个中的1个）、黑色素瘤（4个中的1个）、肺癌（3个中的3个）和白血病（14个中的2个）细胞系。然而，KP-NAC8不与任何检测的横纹肌肉瘤（4个中的0个）、肾母细胞瘤（4个中的0个）、视网膜母细胞瘤（2个中的0个）、神经胶质瘤（4个中的0个）和胃癌（2个中的0个）细胞系结合。在胎儿组织中，KP-NAC8不与5个胎儿肾上腺中的正常神经母细胞反应。在进一步的研究中，通过包括KP-NAC8在内一组12种单克隆抗体分析了胎儿肾上腺神经母细胞的膜表型。将同一组抗体与新鲜NB的结合情况进行比较发现，抗体UJ13A、UJ127:11、PI153/3、抗Thy-1、A2B5、BA-1、BA-2、HSAN1.2和Leu-7与胎儿肾上腺神经母细胞和NB细胞都结合。单克隆抗体OKIa-1和J5不与任何一种组织结合。唯一能够区分胎儿肾上腺神经母细胞和NB细胞的抗体是KP-NAC8。因此，KP-NAC8可能定义了一种与分化相关的抗原，这可能有助于理解NB和原位NB的生物学性质。
