Department of Preventive Medicine, School of public health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
Department of Preventive Medicine, School of public health, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
Toxicol Appl Pharmacol. 2022 Aug 15;449:116097. doi: 10.1016/j.taap.2022.116097. Epub 2022 Jun 6.
Beryllium and its compounds can cause pulmonary interstitial fibrosis through mechanisms that are not yet clear. Long non-coding RNA (lncRNA) is implicated in various diseases. The molecular toxicity of beryllium sulfate (BeSO) was investigated through the RNA-seq analysis of the lncRNA and mRNA whole-transcriptome of BeSO-treated 16HBE cells. A total of 1014 lncRNAs (535 upregulated and 479 downregulated) and 4035 mRNAs (2224 upregulated and 1811 downregulated) were found to be significantly dysregulated (|logFC| ≥> 2.0, p < 0.05) in the BeSO-treated groups when compared with the control group. Five differentially expressed lncRNAs and mRNAs were verified by qRT-PCR. KEGG analysis showed that lncRNA regulates the ECM receiver interaction and PI3K/AKT signaling pathways, etc. In addition, H19:17, lnc-C5orf13-1:1, lnc-CRYAA-17:1, lnc-VSTM5-1:11, and lnc-THSD7A-7:1 may regulate BeSO-induced 16HBE cytotoxicity through ceRNA mechanism. The results of this study will provide some theoretical support for the study of the toxic mechanism of beryllium and its compounds.
铍及其化合物通过尚未明确的机制可引起肺间质纤维化。长链非编码 RNA（lncRNA）与多种疾病有关。本研究通过对硫酸铍（BeSO）处理的 16HBE 细胞的 lncRNA 和 mRNA 全转录组进行 RNA-seq 分析，研究了 BeSO 的分子毒性。与对照组相比，BeSO 处理组的 1014 个 lncRNA（535 个上调和 479 个下调）和 4035 个 mRNA（2224 个上调和 1811 个下调）的表达明显失调（|logFC|≥2.0，p<0.05）。通过 qRT-PCR 验证了 5 个差异表达的 lncRNA 和 mRNA。KEGG 分析表明，lncRNA 调控 ECM 受体相互作用和 PI3K/AKT 信号通路等。此外，H19:17、lnc-C5orf13-1:1、lnc-CRYAA-17:1、lnc-VSTM5-1:11 和 lnc-THSD7A-7:1 可能通过 ceRNA 机制调节 BeSO 诱导的 16HBE 细胞毒性。本研究结果将为研究铍及其化合物的毒性机制提供一些理论支持。
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