Kosowska Katarzyna, Krzysztoforski Jan, Henczka Marek
Faculty of Chemical and Process Engineering, Warsaw University of Technology, Waryńskiego 1, 00-645 Warsaw, Poland.
Materials (Basel). 2022 May 28;15(11):3858. doi: 10.3390/ma15113858.
The process of foaming poly(caprolactone)-based composite materials using supercritical carbon dioxide was analyzed, especially in terms of the biocompatibility of the resultant materials. The influence of foaming process conditions and composite material properties on the functional properties of polymer solid foams, intended for artificial scaffolds for bone cell culture, was investigated. The relationship between wettability (contact angle) and water absorption rate as a result of the application of variable conditions for the production of porous structures was presented. For the evaluation of potential cytotoxicity, the MTT and PrestoBlue tests were carried out, and animal cells (mouse fibroblasts) were cultured on the materials for nine days. There was no toxic effect of composite materials made of poly(caprolactone) containing porogen particles: hydroxyapatite, crystalline nanocellulose, and graphene oxide on cells. The desired effect of the porogens used in the foaming process on the affinity of cells to the resultant material was demonstrated. The tested materials have been shown to be biocompatible and suitable for applications in biomedical engineering.
分析了使用超临界二氧化碳发泡聚己内酯基复合材料的过程，特别是从所得材料的生物相容性方面进行了分析。研究了发泡工艺条件和复合材料性能对用于骨细胞培养人工支架的聚合物固体泡沫功能性能的影响。阐述了由于采用可变条件生产多孔结构而导致的润湿性（接触角）与吸水率之间的关系。为评估潜在的细胞毒性，进行了MTT和PrestoBlue测试，并将动物细胞（小鼠成纤维细胞）在材料上培养九天。含致孔剂颗粒（羟基磷灰石、结晶纳米纤维素和氧化石墨烯）的聚己内酯制成的复合材料对细胞没有毒性作用。证明了发泡过程中使用的致孔剂对细胞与所得材料亲和力的预期效果。测试材料已被证明具有生物相容性，适用于生物医学工程应用。
