将 CRISPR/Cas9 介导的转基因插入到小鼠 Rosa26 安全港的胚胎显微注射。
Microinjection of Zygotes for CRISPR/Cas9-Mediated Insertion of Transgenes into the Murine Rosa26 Safe Harbor.
机构信息
Genome Editing at Macquarie (GEM), Dementia Research Centre, Macquarie University, Sydney, NSW, Australia.
出版信息
Methods Mol Biol. 2022;2495:115-128. doi: 10.1007/978-1-0716-2301-5_7.
Genetically modified (GM) mice are widely used in biomedical research because they can address complex questions in an in-vivo setting that could not otherwise be addressed in-vitro. Microinjection of zygotes remains the most common technique to generate GM animals to date. Here, we describe the targeted insertion (knock-in) of transgenes by microinjection of 1-cell or 2-cell stage embryos into the murine Rosa26 safe harbor.
转基因（GM）小鼠被广泛应用于生物医学研究，因为它们可以在体内环境中解决无法在体外解决的复杂问题。到目前为止，向受精卵进行显微注射仍然是最常见的产生 GM 动物的技术。在这里，我们描述了通过将 1 细胞期或 2 细胞期胚胎显微注射到小鼠 Rosa26 安全港中来进行转基因的靶向插入（基因敲入）。
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