Baytek Gülkiz, Blume Alexander, Demirel Funda Gerceker, Bulut Selman, Popp Oliver, Mertins Philipp, Tursun Baris
Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, 13125, Germany.
Berlin Institute for Medical Systems Biology (BIMSB), Berlin, 10115, Germany.
Biotechniques. 2022 Jun;73(1):5-17. doi: 10.2144/btn-2021-0075. Epub 2022 Jun 14.
Epigenetic mechanisms control chromatin accessibility and gene expression to ensure proper cell fate specification. Histone proteins are integral chromatin components, and their modification promotes gene expression regulation. Specific proteins recognize modified histones such as the chromodomain protein MRG-1. MRG-1 is the ortholog of mammalian MRG15, which is involved in DNA repair. MRG-1 binds methylated histone H3 and is important for germline maturation and safeguarding. To elucidate interacting proteins that modulate MRG-1 activity, we performed in-depth protein-protein interaction analysis using immunoprecipitations coupled with mass spectrometry. We detected strong association with the Small ubiquitin-like modifier SUMO, and found that MRG-1 is post-translationally modified by SUMO. SUMOylation affects chromatin-binding dynamics of MRG-1, suggesting an epigenetic regulation pathway, which may be conserved.
表观遗传机制控制染色质的可及性和基因表达，以确保细胞命运的正确特化。组蛋白是染色质的重要组成部分，其修饰促进基因表达调控。特定蛋白质可识别修饰后的组蛋白，如色域蛋白MRG-1。MRG-1是哺乳动物MRG15的直系同源物，参与DNA修复。MRG-1结合甲基化的组蛋白H3，对生殖系成熟和保护很重要。为了阐明调节MRG-1活性的相互作用蛋白，我们使用免疫沉淀结合质谱进行了深入的蛋白质-蛋白质相互作用分析。我们检测到与小泛素样修饰物SUMO有强烈关联，并发现MRG-1在翻译后被SUMO修饰。SUMO化影响MRG-1的染色质结合动力学，提示了一种可能保守的表观遗传调控途径。
