使用涂覆有 Nafion 的三聚氰胺海绵进行快速灵活的在线脱盐，用于质谱分析。
Rapid and flexible online desalting using Nafion-coated melamine sponge for mass spectrometry analysis.
机构信息
C. Eugene Bennett Department of Chemistry, West Virginia University, Morgantown, WV, USA.
出版信息
Rapid Commun Mass Spectrom. 2022 Sep 15;36(17):e9341. doi: 10.1002/rcm.9341.
RATIONALE
The performance of mass spectrometry (MS) analysis is often affected by the presence of salt ions. To achieve optimal MS detection results, desalting is necessary for samples with high salt concentrations. We report a rapid, low-cost and flexible online desalting method using Nafion-coated sponge. This method is easy to perform and can be implemented to a wide range of customized fluidic systems.
METHODS
Nafion-coated melamine sponge was fabricated by soaking a glass tube containing a melamine sponge in Nafion solution and then drying overnight. The online desalting workflow is comprised of three major parts: (1) Syringe pump, which provides a continuous flow for the online fluid system; (2) Nafion sponge in a glass tube, where the online desalting of sample solution happens; (3) Capillary Vibrating Sharp-Edge Spray Ionization (cVSSI), which is an ionization technique to ionize the desalted analytes.
RESULTS
Effective online desalting of a 10 mM NaCl solution was demonstrated for a wide range of molecules including small molecules, peptides, DNAs, and proteins using a flow rate of 10 μL/min. By incorporating multiple pieces of the Nafion-coated sponge, effective desalting for ubiquitin and cytochrome c (Cyt-c) from physiological buffers, including phosphate-buffered saline (PBS) and tris-buffered saline (TBS), were also achieved. For molecules that are sensitive to low pH conditions after desalting, a R-SO NH -type Nafion-coated sponge was fabricated. Desalting of ubiquitin, oligosaccharide, and DNA oligomers from 10 mM NaCl or 10 mM KCl solutions was demonstrated.
CONCLUSIONS
Flexible, low-cost, and efficient online desalting was achieved by the Nafion-coated sponge. A variety of molecules ranging from small molecules, peptides, proteins to oligosaccharides and DNAs can be desalted for MS analysis. The desalting by Nafion sponge has great potential for desalting applications that require customized fluidic design and rapid analysis.
原理
质谱分析（MS）的性能通常受到盐离子的存在的影响。为了获得最佳的 MS 检测结果，对于高浓度盐的样品需要进行脱盐处理。我们报告了一种使用 Nafion 涂层海绵的快速、低成本和灵活的在线脱盐方法。这种方法易于实施，可以应用于广泛的定制化流体系统。
方法
Nafion 涂层三聚氰胺海绵是通过将含有三聚氰胺海绵的玻璃管浸泡在 Nafion 溶液中并过夜干燥来制备的。在线脱盐工作流程由三个主要部分组成：（1）注射器泵，为在线流体系统提供连续流动；（2）玻璃管中的 Nafion 海绵，样品溶液的在线脱盐在此发生；（3）毛细管振动锐边喷雾电离（cVSSI），这是一种将脱盐分析物离子化的电离技术。
结果
使用 10 μL/min 的流速，成功地对包括小分子、肽、DNA 和蛋白质在内的多种分子的 10 mM NaCl 溶液进行了有效的在线脱盐。通过整合多个 Nafion 涂层海绵，可以有效地从包括磷酸盐缓冲盐水（PBS）和 Tris 缓冲盐水（TBS）在内的生理缓冲液中脱盐泛素和细胞色素 c（Cyt-c）。对于脱盐后对低 pH 条件敏感的分子，制备了 R-SO NH -型 Nafion 涂层海绵。成功地对 10 mM NaCl 或 10 mM KCl 溶液中的泛素、寡糖和 DNA 寡聚物进行了脱盐。
结论
通过 Nafion 涂层海绵实现了灵活、低成本和高效的在线脱盐。可以对从小分子、肽、蛋白质到寡糖和 DNA 的多种分子进行脱盐，用于 MS 分析。Nafion 海绵的脱盐在需要定制化流体设计和快速分析的脱盐应用中具有很大的潜力。
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