College of Chemistry and Chemical Engineering, Yantai University, Yantai 264005, China.
Shandong Dyne Marine Biopharmaceutical Co., Ltd., Weihai 264300, China.
Biosensors (Basel). 2022 May 28;12(6):372. doi: 10.3390/bios12060372.
In this paper, a novel, accurate, sensitive and rapid ratiometric fluorescent sensor was fabricated using a copper nanoclusters@infinite coordination polymer (ICP), specifically, terbium ion-guanosine 5'-disodium (Cu NCs@Tb-GMP) nanocomposites as the ratiometric fluorescent probe, to detect alkaline phosphatase (ALP) in water. The fluorescence probe was characterized by scanning electron microscopy, transmission electron microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. The experimental results showed that, compared with Tb-GMP fluorescent sensors, Cu ratiometric fluorescent sensors based on NCs encapsulated in Tb-GMP had fewer experimental errors and fewer false-positive signals and were more conducive to the sensitive and accurate detection of ALP. In addition, the developed fluorescent probe had good fluorescence intensity, selectivity, repeatability and stability. Under optimized conditions, the ratiometric fluorescent sensor detected ALP in the range of 0.002-2 U mL (R = 0.9950) with a limit of detection of 0.002 U mL, and the recovery of ALP from water samples was less than 108.2%. These satisfying results proved that the ratiometric fluorescent probe has good application prospects and provides a new method for the detection of ALP in real water samples.
本文制备了一种新颖、准确、灵敏、快速的比率荧光传感器，使用铜纳米簇@无限配位聚合物（ICP），具体来说，是铽离子-鸟苷 5'-二钠盐（Cu NCs@Tb-GMP）纳米复合材料作为比率荧光探针，用于检测水中的碱性磷酸酶（ALP）。荧光探针的特征通过扫描电子显微镜、透射电子显微镜、X 射线光电子能谱和傅里叶变换红外光谱进行了表征。实验结果表明，与 Tb-GMP 荧光传感器相比，包埋在 Tb-GMP 中的 Cu 比率荧光传感器具有更少的实验误差和更少的假阳性信号，更有利于 ALP 的灵敏和准确检测。此外，开发的荧光探针具有良好的荧光强度、选择性、重复性和稳定性。在优化条件下，比率荧光传感器在 0.002-2 U mL 范围内检测 ALP（R = 0.9950），检测限为 0.002 U mL，水样中 ALP 的回收率低于 108.2%。这些令人满意的结果证明，比率荧光探针具有良好的应用前景，为实际水样中 ALP 的检测提供了一种新方法。
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