Suzuki Yukako, Hayasaka Ryosuke, Hasebe Masako, Ikeda Satsuki, Soga Tomoyoshi, Tomita Masaru, Hirayama Akiyoshi, Kuroda Hiroki
Institute for Advanced Biosciences, Keio University, Tsuruoka 997-0052, Yamagata, Japan.
Systems Biology Program, Graduate School of Media and Governance, Keio University, Fujisawa 252-0882, Kanagawa, Japan.
Metabolites. 2022 Jun 20;12(6):566. doi: 10.3390/metabo12060566.
Many previous studies have reported the various proteins specifically secreted as inducers in the dorsal or ventral regions in vertebrate gastrula. However, little is known about the effect on cell fate of small molecules below 1000 Da. We therefore tried to identify small molecules specifically expressed in the dorsal marginal zone (DMZ) or ventral marginal zone (VMZ) in vertebrate gastrula. Small intracellular and secreted molecules were detected using explants and supernatant samples. Hydrophilic metabolites were analyzed by capillary ion chromatography-mass spectrometry and liquid chromatography-mass spectrometry, and lipids were analyzed by supercritical fluid chromatography-tandem mass spectrometry. In total, 190 hydrophilic metabolites and 396 lipids were identified. The DMZ was found to have high amounts of glycolysis- and glutathione metabolism-related metabolites in explants, and the VMZ was richer in purine metabolism-related metabolites. We also discovered some hydrophilic metabolites and lipids differentially contained in the DMZ or VMZ. Our research would contribute to a deeper understanding of the cellular physiology that regulates early embryogenesis.
许多先前的研究报告了脊椎动物原肠胚中在背侧或腹侧区域特异性分泌作为诱导剂的各种蛋白质。然而，对于分子量低于1000道尔顿的小分子对细胞命运的影响却知之甚少。因此，我们试图鉴定脊椎动物原肠胚中在背侧边缘区（DMZ）或腹侧边缘区（VMZ）特异性表达的小分子。使用外植体和上清液样本检测细胞内和分泌的小分子。亲水性代谢物通过毛细管离子色谱-质谱联用仪和液相色谱-质谱联用仪进行分析，脂质通过超临界流体色谱-串联质谱进行分析。总共鉴定出190种亲水性代谢物和396种脂质。发现外植体中的DMZ含有大量与糖酵解和谷胱甘肽代谢相关的代谢物，而VMZ富含与嘌呤代谢相关的代谢物。我们还发现了一些在DMZ或VMZ中差异存在的亲水性代谢物和脂质。我们的研究将有助于更深入地理解调节早期胚胎发育的细胞生理学。
