State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China; College of Environment and Ecology, Chongqing University, Chongqing 400044, China.
Chongqing Environment & Sanitation Group, Chongqing 401121, China.
J Hazard Mater. 2022 Aug 15;436:129231. doi: 10.1016/j.jhazmat.2022.129231. Epub 2022 May 26.
The residue derived from oil-based drilling cutting pyrolysis could be used as paving materials. Some petroleum hydrocarbons remain in the residue after pyrolysis and cause severe environmental pollution. In this study, the soil column leaching experiments were carried out under different leaching amounts, and the vertical migration characteristics of petroleum hydrocarbons in soil and the dynamic response mechanism of microorganisms to petroleum hydrocarbons were analyzed. The result showed that the soil pH value and water content with different leaching amounts did not differ significantly, but the vertical migration ability of each petroleum hydrocarbon component was different. In petroleum hydrocarbon contaminated soil, the relative abundance of Proteobacteria maintained a high level (23.6%-60.7%). At the genus level, the relative abundance of Massilia decreased with the leaching amount increased. According to PICRUSt, Monooxygenase [EC: 1.14.13.-] played a significant role in petroleum hydrocarbon degradation. While Long-chain-fatty-acid-CoA ligase [EC: 6.2.1.3] had the highest relative abundance. By studying the influence of shale gas oil-based drilling cuttings pyrolysis residue on soil physical and chemical properties and soil microorganisms, this work provides scientific ecological assessment for the resource application of pyrolysis residue.
油基钻井岩屑热解后的残渣可作为铺路材料。热解后残油中仍含有一些石油烃类物质，会造成严重的环境污染。本研究通过不同淋溶量的土柱淋溶实验，分析石油烃在土壤中的垂向迁移特征及微生物对石油烃的动态响应机制。结果表明，不同淋溶量下土壤 pH 值和含水率无明显差异，但各石油烃组分的垂向迁移能力不同。在石油烃污染土壤中，变形菌门（Proteobacteria）相对丰度保持在较高水平（23.6%-60.7%）。属水平上，随着淋溶量的增加，马西利亚菌属（Massilia）的相对丰度逐渐降低。根据 PICRUSt 预测，单加氧酶[EC:1.14.13.-]在石油烃降解中起重要作用，而长链脂肪酸辅酶 A 连接酶[EC:6.2.1.3]的相对丰度最高。通过研究页岩气油基钻井岩屑热解残渣对土壤理化性质和土壤微生物的影响，为残渣的资源化应用提供了科学的生态评估。
