• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

两性霉素B和安必素的细胞毒性：以及采用鸡胚模型的评估

Cytotoxicity of Amphotericin B and AmBisome: and Evaluation Employing the Chick Embryo Model.

作者信息

Khosravi Ahmad, Sharifi Iraj, Tavakkoli Hadi, Molaakbari Elaheh, Bahraminegad Sina, Salarkia Ehsan, Seyedi Fatemeh, Keyhani Alireza, Salari Zohreh, Sharifi Fatemeh, Bamorovat Mehdi, Afgar Ali, Dabiri Shahriar

机构信息

Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran.

Department of Clinical Science, School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

出版信息

Front Pharmacol. 2022 Jun 8;13:860598. doi: 10.3389/fphar.2022.860598. eCollection 2022.

DOI:10.3389/fphar.2022.860598
PMID:35754489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9214246/
Abstract

Leishmaniasis has been identified as a significant disease in tropical and subtropical regions of the world, with Iran being one of the disease-endemic areas. Various treatments have been applied for this disease, and amphotericin B (Amp B) is the second line of treatment. Side effects of this drug have been reported in various organs. The present study investigated the effects of different types of Amp B on fetal organs using and assays (chicken embryos). analysis was done by checking pathological changes, angiogenesis, and apoptosis alterations on eggs treated by Amp B and AmBisome. approach was employed to predict the affinity of Amp B and AmBisome to the vascular endothelial growth factor A (VEGF-A), its receptor (KDR1), apoptotic-regulator proteins (Bcl-2-associated X protein (Bax), B-cell lymphoma (Bcl-2), and Caspase-8. The ADME-toxicity prediction reveals that AmBisome possesses a superior pharmacological effect to Amp B. The best result of all the dockings in the Molegro Virtual Docker (MVD) was obtained between Bax, Bcl-2, Caspase-8, KDR1, and VEGF-A targets. Due to the lower Egap (HOMO-LUMO) of AmBisome, the chemical reactivity of AmBisome was higher than that of Amp B. analysis showed that embryos that received Amp B exhibited less vascular density than AmBisome. Amp B alone significantly increased the expression of apoptosis and decreased angiogenesis genes compared to AmBisome. The histopathology analysis of the treated embryos showed a reduction in the blood vessel collapse and an increase in degenerative and apoptotic-necrotic changes in the embryonic tissues. Overall, the results suggest the potential benefits of AmBisome over Amp B, which might be a better treatment strategy to treat leishmaniasis during pregnancy.

摘要

利什曼病已被确认为世界热带和亚热带地区的一种重要疾病，伊朗是该疾病的流行地区之一。针对这种疾病已经应用了各种治疗方法，两性霉素B（Amp B）是二线治疗药物。该药物的副作用已在各个器官中被报道。本研究使用[具体实验方法1]和[具体实验方法2]检测（鸡胚），研究了不同类型的两性霉素B对胎儿器官的影响。通过检查经两性霉素B和两性霉素脂质体处理的鸡蛋的病理变化、血管生成和凋亡改变进行[具体分析方法]分析。采用[具体方法]预测两性霉素B和两性霉素脂质体对血管内皮生长因子A（VEGF-A）及其受体（KDR1）、凋亡调节蛋白（Bcl-2相关X蛋白（Bax）、B细胞淋巴瘤（Bcl-2）和半胱天冬酶-8）的亲和力。药物代谢动力学-毒性预测表明，两性霉素脂质体比两性霉素B具有更好的药理作用。在Molegro虚拟对接软件（MVD）中所有对接的最佳结果是在Bax、Bcl-2、半胱天冬酶-8、KDR1和VEGF-A靶点之间获得的。由于两性霉素脂质体的能隙（HOMO-LUMO）较低，其化学反应性高于两性霉素B。[具体分析方法]分析表明，接受两性霉素B处理的胚胎的血管密度低于两性霉素脂质体。与两性霉素脂质体相比，单独使用两性霉素B显著增加了凋亡表达并降低了血管生成基因。对处理后的胚胎进行组织病理学分析显示，血管塌陷减少，胚胎组织中的退行性和凋亡坏死变化增加。总体而言，结果表明两性霉素脂质体相对于两性霉素B具有潜在优势，这可能是孕期治疗利什曼病的更好治疗策略。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/6864a43bcbb7/fphar-13-860598-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/42e744974843/fphar-13-860598-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/143abaa4b1a1/fphar-13-860598-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/43a271a03e88/fphar-13-860598-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/4239487fa00a/fphar-13-860598-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/f39bcf526593/fphar-13-860598-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/98269d0252fd/fphar-13-860598-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/6fc43a13b73a/fphar-13-860598-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/45be03dee830/fphar-13-860598-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/b83dcfb9969e/fphar-13-860598-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/6864a43bcbb7/fphar-13-860598-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/42e744974843/fphar-13-860598-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/143abaa4b1a1/fphar-13-860598-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/43a271a03e88/fphar-13-860598-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/4239487fa00a/fphar-13-860598-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/f39bcf526593/fphar-13-860598-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/98269d0252fd/fphar-13-860598-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/6fc43a13b73a/fphar-13-860598-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/45be03dee830/fphar-13-860598-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/b83dcfb9969e/fphar-13-860598-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71b0/9214246/6864a43bcbb7/fphar-13-860598-g010.jpg

相似文献

1
Cytotoxicity of Amphotericin B and AmBisome: and Evaluation Employing the Chick Embryo Model.两性霉素B和安必素的细胞毒性：以及采用鸡胚模型的评估
Front Pharmacol. 2022 Jun 8;13:860598. doi: 10.3389/fphar.2022.860598. eCollection 2022.
2
Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model.米替福新及其非离子表面活性剂囊泡形式对鸡胚模型细胞毒性的比较。
Sci Rep. 2024 Jan 30;14(1):2482. doi: 10.1038/s41598-024-52620-4.
3
Efficacy and safety of liposomal amphotericin B (AmBisome) for visceral leishmaniasis in endemic developing countries.脂质体两性霉素B（安必素）在地方性流行发展中国家治疗内脏利什曼病的疗效和安全性。
Bull World Health Organ. 1998;76(1):25-32.
4
Post Kala-Azar dermal leishmaniasis following treatment with 20 mg/kg liposomal amphotericin B (Ambisome) for primary visceral leishmaniasis in Bihar, India.在印度比哈尔邦，采用20毫克/千克脂质体两性霉素B（安必素）治疗原发性内脏利什曼病后出现的黑热病后皮肤利什曼病。
PLoS Negl Trop Dis. 2014 Jan 2;8(1):e2611. doi: 10.1371/journal.pntd.0002611. eCollection 2014.
5
Liposomal amphotericin B (AmBisome) for fungal infections in immunocompromised adults and children.脂质体两性霉素B（安必素）用于免疫功能低下的成人和儿童的真菌感染。
Clin Microbiol Infect. 2001;7 Suppl 2:68-79. doi: 10.1111/j.1469-0691.2001.tb00012.x.
6
Pharmacokinetics of liposomal amphotericin B (Ambisome) in critically ill patients.脂质体两性霉素B（安必素）在危重症患者中的药代动力学
Antimicrob Agents Chemother. 1997 Jun;41(6):1275-80. doi: 10.1128/AAC.41.6.1275.
7
Efficacy of liposomal amphotericin B (AmBisome) in the treatment of persistent post-kala-azar dermal leishmaniasis (PKDL).脂质体两性霉素B（安必素）治疗黑热病后皮肤利什曼病（PKDL）的疗效。
Ann Trop Med Parasitol. 2005 Sep;99(6):563-9. doi: 10.1179/136485905X514127.
8
Development of a flow-through USP 4 apparatus drug release assay for the evaluation of amphotericin B liposome.开发一种流通式 USP4 仪器药物释放检测方法，用于评估两性霉素 B 脂质体。
Eur J Pharm Biopharm. 2019 Jan;134:107-116. doi: 10.1016/j.ejpb.2018.11.010. Epub 2018 Nov 24.
9
Comparing the therapeutic efficacy of different amphotericin B-carrying delivery systems against visceral leishmaniasis.比较不同载两性霉素B递送系统治疗内脏利什曼病的疗效。
Exp Parasitol. 2018 Mar;186:24-35. doi: 10.1016/j.exppara.2018.02.003. Epub 2018 Feb 13.
10
Comparison of the physicochemical, antifungal, and toxic properties of two liposomal amphotericin B products.两种脂质体两性霉素B产品的物理化学性质、抗真菌特性及毒性特性比较。
Antimicrob Agents Chemother. 2008 Jan;52(1):259-68. doi: 10.1128/AAC.00870-07. Epub 2007 Oct 29.

引用本文的文献

1
An inclusive assessment of apoptosis mechanisms in species: A narrative literature review.物种凋亡机制的综合评估：一篇叙述性文献综述
Curr Res Parasitol Vector Borne Dis. 2025 Apr 17;7:100260. doi: 10.1016/j.crpvbd.2025.100260. eCollection 2025.
2
The synergistic anti-leishmanial effect of photodynamic therapy employing chemotherapy-mediated nanocomposites.采用化疗介导的纳米复合材料的光动力疗法的协同抗利什曼原虫作用。
Sci Rep. 2025 May 10;15(1):16282. doi: 10.1038/s41598-025-01097-w.
3
Exploring the antifungal potential of -derived stilbenoids and cannabinoids against novel targets through protein interaction profiling.

本文引用的文献

1
Treatment of Complex Cutaneous Leishmaniasis with Liposomal Amphotericin B.脂质体两性霉素B治疗复杂性皮肤利什曼病
Pathogens. 2021 Sep 28;10(10):1253. doi: 10.3390/pathogens10101253.
2
Visceral Leishmaniasis in pregnancy and vertical transmission: A systematic literature review on the therapeutic orphans.妊娠合并内脏利什曼病和垂直传播：治疗孤儿的系统文献综述。
PLoS Negl Trop Dis. 2021 Aug 10;15(8):e0009650. doi: 10.1371/journal.pntd.0009650. eCollection 2021 Aug.
3
Nanotechnology approaches for delivery and targeting of Amphotericin B in fungal and parasitic diseases.
通过蛋白质相互作用谱分析探索源自-的芪类化合物和大麻素对新靶点的抗真菌潜力。
Front Chem. 2025 Jan 6;12:1515424. doi: 10.3389/fchem.2024.1515424. eCollection 2024.
4
MICROBIOME: The trials and errors of developing an experimental model to study the impact of maternal gut microbiome disruption on perinatal asphyxia.微生物组：开发一种实验模型来研究母体肠道微生物组破坏对围产期窒息影响的试验和错误。
Reprod Fertil. 2024 Nov 6;5(4). doi: 10.1530/RAF-24-0050. Print 2024 Oct 1.
5
Comparison of cytotoxicity of Miltefosine and its niosomal form on chick embryo model.米替福新及其非离子表面活性剂囊泡形式对鸡胚模型细胞毒性的比较。
Sci Rep. 2024 Jan 30;14(1):2482. doi: 10.1038/s41598-024-52620-4.
6
In silico and in vitro potentials of crocin and amphotericin B on Leishmania major: Multiple synergistic mechanisms of actions.西红花酸和两性霉素 B 在体外和体内对利什曼原虫的作用潜力：多种协同作用机制。
PLoS One. 2023 Sep 8;18(9):e0291322. doi: 10.1371/journal.pone.0291322. eCollection 2023.
7
Chemical, biological and assessment of date ( L.) fruits grown in Ha'il region.哈伊勒地区种植的椰枣（L.）果实的化学、生物学及评估
Front Chem. 2023 Mar 3;11:1138057. doi: 10.3389/fchem.2023.1138057. eCollection 2023.
8
Empagliflozin induces apoptotic-signaling pathway in embryonic vasculature: and approaches chick's yolk sac membrane model.恩格列净诱导胚胎血管中的凋亡信号通路：并采用鸡卵黄囊膜模型。
Front Pharmacol. 2022 Sep 1;13:970402. doi: 10.3389/fphar.2022.970402. eCollection 2022.
纳米技术在真菌和寄生虫病中用于两性霉素 B 的递送和靶向的应用。
Nanomedicine (Lond). 2021 Apr;16(10):857-877. doi: 10.2217/nnm-2020-0482. Epub 2021 Apr 23.
4
Sixty years of Amphotericin B: An Overview of the Main Antifungal Agent Used to Treat Invasive Fungal Infections.两性霉素B的六十年：用于治疗侵袭性真菌感染的主要抗真菌药物综述
Infect Dis Ther. 2021 Mar;10(1):115-147. doi: 10.1007/s40121-020-00382-7. Epub 2021 Feb 1.
5
The Problem with Amphotericin.两性霉素的问题。
Clin Drug Investig. 2020 Aug;40(8):687-693. doi: 10.1007/s40261-020-00924-4.
6
Old world cutaneous leishmaniasis in Iran: clinical variants and treatments.伊朗的旧世界皮肤利什曼病：临床变种与治疗方法
J Dermatolog Treat. 2021 Nov;32(7):673-683. doi: 10.1080/09546634.2019.1704214. Epub 2020 Jan 8.
7
Vascular alteration in relation to fosfomycine: In silico and in vivo investigations using a chick embryo model.与膦甲酸相关的血管改变：使用鸡胚模型的体内和体外研究。
Biomed Pharmacother. 2019 Oct;118:109240. doi: 10.1016/j.biopha.2019.109240. Epub 2019 Aug 8.
8
Host's immune response in unresponsive and responsive patients with anthroponotic cutaneous leishmaniasis treated by meglumine antimoniate: A case-control study of Th1 and Th2 pathways.宿主对米氮平治疗的人源化皮肤利什曼病无反应和有反应患者的免疫反应：Th1 和 Th2 途径的病例对照研究。
Int Immunopharmacol. 2019 Apr;69:321-327. doi: 10.1016/j.intimp.2019.02.008. Epub 2019 Feb 14.
9
Toxico-pathological effects of meglumine antimoniate on human umbilical vein endothelial cells.米诺环素对人脐静脉内皮细胞的毒理病理作用。
Toxicol In Vitro. 2019 Apr;56:10-18. doi: 10.1016/j.tiv.2018.12.018. Epub 2018 Dec 30.
10
A single-group trial of end-stage patients with anthroponotic cutaneous leishmaniasis: Levamisole in combination with Glucantime in field and laboratory models.一单组临床试验：针对人源化皮肤利什曼病的终末期患者：在野外和实验室模型中联合使用左旋咪唑和葡萄糖酸锑钠。
Microb Pathog. 2019 Mar;128:162-170. doi: 10.1016/j.micpath.2018.12.040. Epub 2018 Dec 21.