Blanck A, Wicksell L, Eriksson L C
Toxicol Pathol. 1987;15(1):103-5. doi: 10.1177/019262338701500115.
Hepatocyte nodules produced by cyclic feeding of the rat liver carcinogen 2-acetylaminofluorene to male rats have been shown to exhibit specific metabolic characteristics. None of the characteristics of nodular tissue so far investigated has provided any explanation why initiated cells, such as in the "resistant hepatocyte model," were resistant to the mitoinhibitory effects of 2-acetylaminofluorene. In the present study, hepatocyte nodules are produced by cyclic feeding of 0.05% 2-AAF in the diet to male Wistar rats. N-hydroxy-2-acetylaminofluorene sulfotransferase levels were determined in the postmicrosomal supernatant prepared from nodules and from control rat liver. The average sulfotransferase activities were 0.29 and 4.75 nmol p-nitrophenol formed/min X mg protein, respectively. We suggest that the low sulfotransferase activity in preparations from nodular tissue might be an important part of the explanation for the resistance of focal and nodular tissue to the mitoinhibitory effects of 2-acetylaminofluorene.
给雄性大鼠周期性喂食肝脏致癌物2-乙酰氨基芴所产生的肝细胞结节已被证明具有特定的代谢特征。迄今为止所研究的结节组织的特征，均无法解释为何像“抗性肝细胞模型”中的起始细胞对2-乙酰氨基芴的有丝分裂抑制作用具有抗性。在本研究中，通过在饮食中给雄性Wistar大鼠周期性喂食0.05%的2-乙酰氨基芴来产生肝细胞结节。测定了从结节和对照大鼠肝脏制备的微粒体后上清液中N-羟基-2-乙酰氨基芴磺基转移酶的水平。平均磺基转移酶活性分别为每分钟每毫克蛋白质形成0.29和4.75纳摩尔对硝基苯酚。我们认为，结节组织制剂中低磺基转移酶活性可能是局灶性和结节性组织对2-乙酰氨基芴有丝分裂抑制作用具有抗性的重要解释之一。
