Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, Hefei 230038, China; Key Laboratory of Xin'an Medicine, Ministry of Education, Anhui University of Chinese Medicine, Hefei 230038, China.
Anhui University of Chinese Medicine and Anhui Academy of Chinese Medicine, Hefei 230038, China.
Food Res Int. 2022 Jul;157:111375. doi: 10.1016/j.foodres.2022.111375. Epub 2022 May 14.
Camellia sinensis (L.) O. Kuntze is used to produce tea, a beverage consumed worldwide. Catechins are major medically active components of C. sinensis and can be used clinically to treat hyperglycaemia, hypertension, and cancer. In this study, we aimed to identify the genes involved in catechins biosynthesis. To this end, we analysed transcriptome data from two different cultivars of C. sinensis using DNBSEQ technology. In total,47,717 unigenes were obtained from two cultivars of C. sinensis, of which 9429 were predicted as new unigenes. In our analyses of the Kyoto Encyclopedia of Genes and Genomes database, 212 unigenes encoding 13 key enzymes involved in catechins biosynthesis were identified; the structures of leucoanthocyanidin reductase and anthocyanidin reductase were spatially modelled. Some of these key enzymes were verified by real-time quantitative polymerase chain reaction, and multiple genes encoding plant resistance proteins or transcription factors were identified and analysed. Furthermore, two microRNAs involved in the regulation of catechins biosynthesis were explored. Differentially expressed genes involved in the flavonoid biosynthesis pathway were identified from pairwise comparisons of genes from different cultivars of tea plants. Overall, our findings expanded the number of publicly available transcript datasets for this valuable plant species and identified candidate genes related to the biosynthesis of C. sinensis catechins, thereby establishing a foundation for further in-depth studies of catechins biosynthesis in varieties or cultivars of C. sinensis.
茶树（Camellia sinensis (L.) O. Kuntze）被用于生产茶叶，这是一种在全球范围内消费的饮料。儿茶素是茶树的主要药用活性成分，可用于临床治疗高血糖、高血压和癌症。在本研究中，我们旨在鉴定儿茶素生物合成相关的基因。为此，我们使用 DNBSEQ 技术分析了来自两个不同茶树品种的转录组数据。总共从两个茶树品种中获得了 47717 条非编码 RNA ，其中 9429 条被预测为新的非编码 RNA 。在对京都基因与基因组百科全书数据库的分析中，鉴定了 212 条编码 13 种儿茶素生物合成关键酶的非编码 RNA ，并对其结构进行了空间建模。这些关键酶中的一些通过实时定量聚合酶链反应得到了验证，并鉴定和分析了多个编码植物抗性蛋白或转录因子的基因。此外，还研究了两种参与儿茶素生物合成调控的 microRNA 。通过茶树不同品种基因的两两比较，鉴定了参与类黄酮生物合成途径的差异表达基因。总之，我们的研究结果扩展了该有价值植物物种的公开转录组数据集的数量，并鉴定了与茶树儿茶素生物合成相关的候选基因，为进一步深入研究茶树品种或品种儿茶素生物合成奠定了基础。
