Data for quantitative research of mechanical properties of agar media with concentration gradient, and root growth in these media.

The mechanical properties of the plant culture medium affect plant growth and development significantly. The paper presents the data created for the published article entitled "Resistance from agar medium impacts the helical growth of primary roots". The data contains the real-time output forces of 0.5‒1.2% agar media from Bluehill software, and the forces on the agar surfaces changing with the increase of displacement. Oscillatory rheological experiments were employed to verify the stiffness results of 0.5‒1.2% agar media. Helix diameter and length of roots grown in gradient agar media for Col-0 and DR5-GUS are exhibited.