Mahmod S, Smith D S, Landon J
Ther Drug Monit. 1987;9(1):91-6. doi: 10.1097/00007691-198703000-00016.
A robust commercial radioimmunoassay kit for serum digoxin was adapted to salivary assay by simple procedural modifications and shown to have satisfactory sensitivity, precision, accuracy, and specificity. The digoxin saliva/serum concentration ratio in 50 hospital patients was 0.67 +/- 0.10 (mean +/- SD), and the levels were well correlated (r = 0.96). Similar saliva/serum ratios were found in two normal subjects who took digoxin orally, and the free fraction in serum, determined by equilibrium dialysis, was found to equate with the salivary drug level. Digoxin appeared in saliva at its maximum level within 1 min of intravenous injection. Although previous studies of salivary digoxin have given widely discrepant results, the present findings are entirely consistent with the simple expectation that digoxin should appear in saliva by passive diffusion. Salivary sampling is suitable for the monitoring of digoxin therapy and pharmacokinetics, and the modified radioimmunoassay should be of value to laboratories wishing to introduce this technique.
一种用于血清地高辛的强大商业放射免疫分析试剂盒，通过简单的程序修改适用于唾液检测，并显示出具有令人满意的灵敏度、精密度、准确度和特异性。50名住院患者的地高辛唾液/血清浓度比为0.67±0.10（平均值±标准差），且两者水平具有良好的相关性（r = 0.96）。在两名口服地高辛的正常受试者中也发现了类似的唾液/血清比，通过平衡透析测定的血清中游离部分与唾液中的药物水平相当。静脉注射后1分钟内地高辛在唾液中达到最高水平。尽管先前关于唾液地高辛的研究结果差异很大，但目前的发现完全符合地高辛应通过被动扩散出现在唾液中的简单预期。唾液采样适用于地高辛治疗和药代动力学监测，改良的放射免疫分析对希望引入该技术的实验室应该有价值。
