建立一个多重成像面板，以研究小鼠胸腺中 T 细胞的发育。

Establishing a multiplex imaging panel to study T cell development in the thymus in mouse.

机构信息

Optical Sciences Centre, School of Science Computing and Engineering Technologies, Swinburne University of Technology, Hawthorn, VIC 3122, Australia; Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia; Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia.

Olivia Newton-John Cancer Research Institute and La Trobe University School of Cancer Medicine, Heidelberg, VIC, 3084, Australia; Immune Signalling Laboratory, Peter MacCallum Cancer Centre, Melbourne, VIC 3000 Australia; Sir Peter MacCallum Department of Oncology, The University of Melbourne, Parkville, VIC 3000, Australia.

出版信息

STAR Protoc. 2022 Jun 21;3(3):101472. doi: 10.1016/j.xpro.2022.101472. eCollection 2022 Sep 16.

DOI:10.1016/j.xpro.2022.101472

PMID:35769925

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9234470/

Abstract

Multiplexed immunohistochemistry enables analysis of cellular and signaling events in the context of an intact organ. Here, we describe protocols for applying multiplexed immunohistochemistry to the mouse thymus. In particular, we describe how to identify cells at the specific differentiation stage known as β-selection, and to monitor pre-TCR signaling and the cellular response at that stage. For complete details on the use and execution of this protocol, please refer to Allam et al. (2021).

摘要

多重免疫组化使分析细胞和信号事件在一个完整的器官的背景下成为可能。在这里，我们描述了将多重免疫组化应用于小鼠胸腺的方案。特别是，我们描述了如何鉴定特定分化阶段（称为β选择）的细胞，并监测该阶段的 pre-TCR 信号和细胞反应。有关此方案的使用和执行的完整详细信息，请参阅 Allam 等人。（2021）。