Zhao Le, Zhu Yunhao, Wang Min, Han Yongguang, Xu Jiao, Feng Weisheng, Zheng Xiaoke
School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou, China.
Co-construction Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases by Henan and Education Ministry of P.R. China, Zhengzhou, China.
Int J Phytoremediation. 2023;25(5):562-571. doi: 10.1080/15226514.2022.2092064. Epub 2022 Jul 8.
is a Cd hyperaccumulator plant that accumulates significant amounts of Cd in leaves, making it a valuable phytoremediation plant species. Our previous research found enolase (ENO) may play an important part in to cope with Cd stress. As a multifunctional enzyme, ENO was involved not only in glycolysis but also in the response of plants to various environmental stresses. However, there are few studies on the function of PaENO ( enolase) in coping with Cd stress. In this study, the gene was isolated from , and the expression level of gene significantly increased after Cd treatment. The enzymatic activity analysis showed PaENO had typical ENO activity, and the 42-position serine was essential to the enzymatic activity of PaENO. The Cd resistance assay indicated the expression of PaENO remarkably enhanced the resistance of to Cd, which was achieved by reducing the Cd content in . Moreover, both the expression of inactive PaENO and PaMBP-1 (alternative translation product of PaENO) can improve the tolerance of to Cd. The results indicated PaENO may be alternatively translated into the transcription factor PaMBP-1 to participate in the response of to Cd stress.
是一种镉超积累植物，其叶片中积累大量镉，使其成为一种有价值的植物修复植物物种。我们之前的研究发现烯醇化酶（ENO）可能在应对镉胁迫中起重要作用。作为一种多功能酶，ENO不仅参与糖酵解，还参与植物对各种环境胁迫的反应。然而，关于PaENO（烯醇化酶）在应对镉胁迫中的功能研究较少。在本研究中，从 中分离出 基因，镉处理后 基因的表达水平显著增加。酶活性分析表明PaENO具有典型的ENO活性，42位丝氨酸对PaENO的酶活性至关重要。镉抗性试验表明，PaENO的表达显著增强了 对镉的抗性，这是通过降低 中的镉含量实现的。此外，无活性的PaENO和PaMBP-1（PaENO的可变翻译产物）的表达都可以提高 对镉的耐受性。结果表明，PaENO可能被可变翻译成转录因子PaMBP-1，参与 对镉胁迫的反应。
