内源性脱氧核糖核酸酶活性的变异性及其病理生理学后果。

Variability of endogenous deoxyribonuclease activity and its pathophysiological consequences.

机构信息

Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia.

Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia; Center for Biomedical Technology, Department for Health Sciences and Biomedicine, Danube University, 3500, Krems, Austria.

出版信息

Mol Cell Probes. 2022 Oct;65:101844. doi: 10.1016/j.mcp.2022.101844. Epub 2022 Jul 5.

DOI:10.1016/j.mcp.2022.101844

PMID:35803442

Abstract

Deoxyribonucleases (DNases) are enzymes that cleave DNA. Some DNases are secreted outside of cells where they can cleave extracellular DNA (ecDNA). High concentration of ecDNA is associated with diseases such as sepsis, preeclampsia, and systemic lupus. DNA can be released from dying cells and is immunogenic. DNases cleave ecDNA and might prevent activation of the immune system. Low DNase activity could be disadvantageous in diseases where high amounts of ecDNA are released from dying cells. The relationship between DNase activity and ecDNA remains unknown. The lack of standard values in DNase activity makes the studies difficult to compare. This review focuses on summarizing methods for DNase activity measurements, the possible implication of decreased DNase activity in diseases, and the impact on diseases associated with a high concentration of ecDNA.

摘要

脱氧核糖核酸酶（DNases）是能够切割 DNA 的酶。一些 DNases 会分泌到细胞外，在那里它们可以切割细胞外 DNA（ecDNA）。高浓度的 ecDNA 与败血症、先兆子痫和系统性红斑狼疮等疾病有关。DNA 可以从死亡的细胞中释放出来，具有免疫原性。DNases 可以切割 ecDNA，从而可能阻止免疫系统的激活。在大量 ecDNA 从死亡细胞中释放的疾病中，低 DNase 活性可能是不利的。DNase 活性与 ecDNA 之间的关系尚不清楚。DNase 活性缺乏标准值使得这些研究难以进行比较。这篇综述重点总结了 DNase 活性测量的方法、DNase 活性降低在疾病中的可能影响，以及对与高浓度 ecDNA 相关的疾病的影响。