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通过超短激光纹理化优化丝素薄膜的表面结构和形态特性以创建肌肉细胞基质模型。

Optimizing the Surface Structural and Morphological Properties of Silk Thin Films via Ultra-Short Laser Texturing for Creation of Muscle Cell Matrix Model.

作者信息

Angelova Liliya, Daskalova Albena, Filipov Emil, Vila Xavier Monforte, Tomasch Janine, Avdeev Georgi, Teuschl-Woller Andreas H, Buchvarov Ivan

机构信息

Institute of Electronics, Bulgarian Academy of Sciences, 72 Tzarigradsko Shousse Blvd., 1784 Sofia, Bulgaria.

Department Life Science Engineering, University of Applied Sciences Technikum Wien, Höchstädtplatz 6, 1200 Vienna, Austria.

出版信息

Polymers (Basel). 2022 Jun 25;14(13):2584. doi: 10.3390/polym14132584.

DOI:10.3390/polym14132584
PMID:35808630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9269134/
Abstract

Temporary scaffolds that mimic the extracellular matrix's structure and provide a stable substratum for the natural growth of cells are an innovative trend in the field of tissue engineering. The aim of this study is to obtain and design porous 2D fibroin-based cell matrices by femtosecond laser-induced microstructuring for future applications in muscle tissue engineering. Ultra-fast laser treatment is a non-contact method, which generates controlled porosity-the creation of micro/nanostructures on the surface of the biopolymer that can strongly affect cell behavior, while the control over its surface characteristics has the potential of directing the growth of future muscle tissue in the desired direction. The laser structured 2D thin film matrices from silk were characterized by means of SEM, EDX, AFM, FTIR, Micro-Raman, XRD, and 3D-roughness analyses. A WCA evaluation and initial experiments with murine C2C12 myoblasts cells were also performed. The results show that by varying the laser parameters, a different structuring degree can be achieved through the initial lifting and ejection of the material around the area of laser interaction to generate porous channels with varying widths and depths. The proper optimization of the applied laser parameters can significantly improve the bioactive properties of the investigated 2D model of a muscle cell matrix.

摘要

模仿细胞外基质结构并为细胞自然生长提供稳定基质的临时支架是组织工程领域的一种创新趋势。本研究的目的是通过飞秒激光诱导微结构化来获得和设计基于二维丝素蛋白的多孔细胞基质，以供未来在肌肉组织工程中应用。超快激光处理是一种非接触方法，它能产生可控的孔隙率——在生物聚合物表面形成微/纳米结构，这会强烈影响细胞行为，而对其表面特性的控制有可能引导未来肌肉组织朝着期望的方向生长。通过扫描电子显微镜（SEM）、能谱仪（EDX）、原子力显微镜（AFM）、傅里叶变换红外光谱仪（FTIR）、显微拉曼光谱仪、X射线衍射仪（XRD）和三维粗糙度分析对激光结构化丝素二维薄膜基质进行了表征。还进行了水接触角（WCA）评估以及对小鼠C2C12成肌细胞的初步实验。结果表明，通过改变激光参数，可通过激光作用区域周围材料的初始提升和喷射来实现不同的结构化程度，从而生成具有不同宽度和深度的多孔通道。对所应用激光参数的适当优化可显著改善所研究的肌肉细胞基质二维模型的生物活性特性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9ef6/9269134/151e9e06d9e1/polymers-14-02584-g011.jpg
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本文引用的文献

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Polymers (Basel). 2021 Aug 3;13(15):2577. doi: 10.3390/polym13152577.
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