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在来自厄瓜多尔的玉米和约翰逊草中发现了两种新的类伞形病毒相关RNA(ulaRNA)。

Two new umbravirus-like associated RNAs (ulaRNAs) discovered in maize and johnsongrass from Ecuador.

作者信息

Quito-Avila Diego F, Reyes-Proaño Edison G, Mendoza Alma, Margaria Paolo, Menzel Wulf, Bera Sayanta, Simon Anne E

机构信息

Facultad de Ciencias de la Vida, Escuela Superior Politécnica del Litoral, ESPOL, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador.

Centro de Investigaciones Biotecnológicas del Ecuador, CIBE, Escuela Superior Politécnica del Litoral, Km 30.5 Vía Perimetral Campus Gustavo Galindo, Guayaquil, Ecuador.

出版信息

Arch Virol. 2022 Oct;167(10):2093-2098. doi: 10.1007/s00705-022-05525-4. Epub 2022 Jul 12.

Abstract

Two new umbravirus-like associated RNAs (ulaRNAs) were found, respectively, in maize and Johnsongrass samples from Ecuador. The complete sequences consist of 3,053 and 3,025 nucleotides, respectively, and contain four open reading frames (ORFs). Their genome sequences were 58% identical to each other and 28 to 60% identical to the most closely related viruses. Phylogenetic analysis using full genome sequences and amino acid sequence of the RNA-dependent-RNA polymerase (RdRp) placed both sequences in a clade sharing the most recent common ancestor with ulaRNAs from sugarcane and maize, suggesting that they belong to a monophyletic grass-infecting lineage. Their terminal regions exhibit features common to umbraviruses and ulaRNAs.

摘要

分别在来自厄瓜多尔的玉米和约翰逊草样本中发现了两种新的类伞形病毒相关RNA(ulaRNAs)。完整序列分别由3053和3025个核苷酸组成,并包含四个开放阅读框(ORF)。它们的基因组序列彼此间有58%的同一性,与亲缘关系最近的病毒有28%至60%的同一性。使用全基因组序列和RNA依赖性RNA聚合酶(RdRp)的氨基酸序列进行的系统发育分析将这两个序列置于一个与来自甘蔗和玉米的ulaRNAs共享最近共同祖先的分支中,这表明它们属于一个单系的感染禾本科植物的谱系。它们的末端区域呈现出伞形病毒和ulaRNAs共有的特征。

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