Ahmad Enas Sebaey, Diab Safia Mohamed, Behiry Eman Gamal, Bassyoni Shereen El Bassyoni El Said El, Ishak Sally Raafat, Ramadan Abeer
Faculty of Medicine, Benha University, Benha, Egypt.
Faculty of Medicine, Tanta University, Tanta, Egypt.
Pediatr Pulmonol. 2022 Nov;57(11):2659-2664. doi: 10.1002/ppul.26079. Epub 2022 Aug 3.
Bronchial asthma is a common chronic respiratory disease in children with complex pathogenesis, characterized by airway hyper-responsiveness, obstruction, mucus hyperproduction, and airway remodeling. Autophagy is important for cellular physiology, and the ATG5 rs510432 has recently been implicated in several fundamental characteristics of childhood asthma pathogenesis and may play a role in the disease progression. This study aims to assess the expression of ATG5 messenger RNA (mRNA) according to rs510432 polymorphism in asthmatic children and to evaluate their possible relation with the development of the disease.
ATG5 mRNA expression and rs510432 polymorphism were measured using real-time polymerase chain reaction in 57 asthmatic children patients and 46 healthy controls.
ATG5 level was significantly higher in asthmatic children than in controls and a significant increase in the frequency of TT and CC genotype of ATG5 rs510432 gene polymorphism was found in asthmatic patients when compared to control subjects (p < 0.001; and p = 0.01, respectively), and there was a statistically significant decrease in the frequency of CT genotype of ATG5 rs510432 gene polymorphism in asthmatic patients when compared to control subjects (p < 0.001).
ATG5 rs510432 gene polymorphism plays an important role in childhood asthma pathogenesis.
支气管哮喘是儿童常见的慢性呼吸道疾病，发病机制复杂，其特征为气道高反应性、阻塞、黏液过度分泌及气道重塑。自噬对细胞生理功能很重要，近期研究表明自噬相关基因5（ATG5）的rs510432与儿童哮喘发病机制的几个基本特征有关，可能在疾病进展中发挥作用。本研究旨在评估哮喘儿童中根据rs510432多态性的ATG5信使核糖核酸（mRNA）表达，并评估其与疾病发展的可能关系。
采用实时聚合酶链反应检测57例哮喘儿童患者和46例健康对照者的ATG5 mRNA表达及rs510432多态性。
哮喘儿童的ATG5水平显著高于对照组，与对照组相比，哮喘患者中ATG5 rs510432基因多态性的TT和CC基因型频率显著增加（分别为p＜0.001和p = 0.01），与对照组相比，哮喘患者中ATG5 rs510432基因多态性的CT基因型频率有统计学意义的降低（p＜0.001）。
ATG5 rs510432基因多态性在儿童哮喘发病机制中起重要作用。
