利用宋内志贺菌外膜蛋白建立基于抗体的免疫检测方法诊断志贺菌病的价值。

Usefulness of the outer membrane proteins of Shigella sonnei in developing an antibody-based immunoassay for the diagnosis of shigellosis.

机构信息

Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, Malaysia.

School of Pharmaceutical Sciences, Universiti Sains Malaysia, Penang, Malaysia.

出版信息

Trop Biomed. 2022 Jun 1;39(2):252-256. doi: 10.47665/tb.39.2.015.

DOI:10.47665/tb.39.2.015

PMID:35838099

Abstract

Conventional culture method and biochemical tests remain as the 'gold standard' method for the identification of S. sonnei which are time-consuming. We have discovered previously the potential of three OMPs of S. sonnei (33.3 kDa, 43.8 kDa and 100.3 kDa) as biomarkers in the diagnostic test for shigellosis. Here, we evaluated the performance of the outer membrane proteins of S. sonnei for the development of an antibody-based immunoassay for the detection of S. sonnei infections. All threetarget proteins were specifically recognized when probed with S. sonnei sera. In addition, another two potential proteins of molecular weight 29.0 kDa and 88.2 kDa in size were also exclusively recognized by the IgA when probed with S. sonnei sera. The optimized ELISA demonstrated higher sensitivity and specificity which exceeded 86.0%. In conclusion, the identified target proteins showed great potential as diagnostic biomarkers for the detection of S. sonnei infections in patients.

摘要

传统的培养方法和生化试验仍然是志贺氏菌属鉴定的“金标准”方法，但耗时较长。我们之前发现志贺氏菌属的三种外膜蛋白（33.3 kDa、43.8 kDa 和 100.3 kDa）具有作为志贺菌病诊断试验生物标志物的潜力。在这里，我们评估了志贺氏菌属外膜蛋白在开发基于抗体的免疫检测以检测志贺氏菌属感染方面的性能。当用志贺氏菌属血清探测时，所有三种靶蛋白都被特异性识别。此外，当用志贺氏菌属血清探测时，另两种潜在的分子量为 29.0 kDa 和 88.2 kDa 的蛋白质也被 IgA 特异性识别。优化后的 ELISA 显示出超过 86.0%的更高灵敏度和特异性。总之，所鉴定的靶蛋白显示出作为检测患者志贺氏菌属感染的诊断生物标志物的巨大潜力。