Wilson H C, Milos N C
In Vitro Cell Dev Biol. 1987 May;23(5):323-31. doi: 10.1007/BF02620988.
This study investigates the nutritional requirements of Xenopus laevis neural crest cells and melanophores developing in vitro. A comparison is made between the growth and differentiation of cells in serum-containing medium and a chemically defined, serum-free medium that we have designed. Our chemically defined medium is more efficient than serum-supplemented medium in promoting proliferation of these cells. Several supplements are required to enhance culture development. These include insulin, alpha-melanocyte stimulating hormone, somatotropin, luteotrophic hormone, linoleic acid, uridine, and putrescine. In addition, collagen and fibronectin provide the most conducive environment tested for cell migration and adhesion.
本研究调查了非洲爪蟾神经嵴细胞和体外发育的黑素细胞的营养需求。对含血清培养基和我们设计的化学成分明确的无血清培养基中细胞的生长和分化进行了比较。我们的化学成分明确的培养基在促进这些细胞增殖方面比补充血清的培养基更有效。需要几种补充剂来促进培养发展。这些包括胰岛素、α-黑素细胞刺激素、生长激素、促黄体激素、亚油酸、尿苷和腐胺。此外，胶原蛋白和纤连蛋白为测试细胞迁移和黏附提供了最有利的环境。
