镁通道和转运蛋白的结构生物学研究进展。
Recent Advances in the Structural Biology of Mg Channels and Transporters.
机构信息
State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Neurobiology, School of Life Sciences, Fudan University, Shanghai 200438, China.
State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Bioactive Small Molecules, Collaborative Innovation Center of Genetics and Development, Department of Physiology and Neurobiology, School of Life Sciences, Fudan University, Shanghai 200438, China.
出版信息
J Mol Biol. 2022 Oct 15;434(19):167729. doi: 10.1016/j.jmb.2022.167729. Epub 2022 Jul 13.
Magnesium ions (Mg) are the most abundant divalent cations in living organisms and are essential for various physiological processes, including ATP utilization and the catalytic activity of numerous enzymes. Therefore, the homeostatic mechanisms associated with cellular Mg are crucial for both eukaryotic and prokaryotic organisms and are thus strictly controlled by Mg channels and transporters. Technological advances in structural biology, such as the expression screening of membrane proteins, in meso phase crystallization, and recent cryo-EM techniques, have enabled the structure determination of numerous Mg channels and transporters. In this review article, we provide an overview of the families of Mg channels and transporters (MgtE/SLC41, TRPM6/7, CorA/Mrs2, CorC/CNNM), and discuss the structural biology prospects based on the known structures of MgtE, TRPM7, CorA and CorC.
镁离子（Mg）是生物体内最丰富的二价阳离子，对各种生理过程至关重要，包括 ATP 的利用和许多酶的催化活性。因此，与细胞镁相关的动态平衡机制对真核生物和原核生物都至关重要，因此受到镁通道和转运蛋白的严格控制。结构生物学的技术进步，如膜蛋白的表达筛选、中相结晶和最近的冷冻电镜技术，使众多镁通道和转运蛋白的结构得以确定。在这篇综述文章中，我们概述了镁通道和转运蛋白家族（MgtE/SLC41、TRPM6/7、CorA/Mrs2、CorC/CNNM），并根据已知的 MgtE、TRPM7、CorA 和 CorC 结构讨论了结构生物学的前景。
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