Suppr超能文献

单细胞转录组揭示司美格鲁肽对肥胖小鼠非心肌细胞的影响。

Single-cell transcriptome reveals effects of semaglutide on non-cardiomyocytes of obese mice.

机构信息

Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, China; Department of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei, China.

Department of Internal Medicine, Hebei Medical University, Shijiazhuang, Hebei, China; Department of Nephrology, Hebei General Hospital, Shijiazhuang, Hebei, China.

出版信息

Biochem Biophys Res Commun. 2022 Sep 24;622:22-29. doi: 10.1016/j.bbrc.2022.07.034. Epub 2022 Jul 9.

Abstract

Non-cardiomyocytes (nonCMs) play an important part in cardiac fibrosis pathophysiology, but the underlying molecular pathways are unknown. Semaglutide has cardioprotective properties, but it is still unclear whether it helps with cardiac fibrosis and what the processes are. The goal of this study is to use single cell transcriptomics approaches to investigate the molecular mechanism of semaglutide's cardioprotective action in obese mice. We found 15 non-CMs, with fibroblasts making up the majority of them. We found eight DEGs that altered significantly following semaglutide treatment by screening for differentially expressed genes (DEGs). DEGs were shown to have biological activities primarily related to extracellular matrix and collagen synthesis and distribution, with Serpinh1 and Pcolce expression being the most dramatically altered. Serpinh1 and Pcolce were mostly found in fibroblasts, which play a key role in the fibrosis of the heart. Furthermore, we discovered that semaglutide lowered cardiac collagen content and alleviated obesity-induced ventricular wall hypertrophy. As a result, our findings show that Serpinh1 and Pcolce, which are expressed by fibroblasts, may play a role in the development of obese cardiac fibrosis. By reducing Serpinh1 and Pcolce expression and delaying cardiac fibrosis, semaglutide may have a cardioprotective effect.

摘要

非心肌细胞(nonCMs)在心脏纤维化病理生理学中起着重要作用,但潜在的分子途径尚不清楚。司美格鲁肽具有心脏保护特性,但它是否有助于心脏纤维化以及其作用过程仍不清楚。本研究旨在使用单细胞转录组学方法来研究肥胖小鼠中司美格鲁肽的心脏保护作用的分子机制。我们发现了 15 种非心肌细胞,其中成纤维细胞占大多数。通过筛选差异表达基因(DEGs),我们发现了 8 个在司美格鲁肽治疗后明显改变的 DEGs。DEGs 表现出的生物学活性主要与细胞外基质和胶原合成与分布有关,Serpinh1 和 Pcolce 的表达变化最为显著。Serpinh1 和 Pcolce 主要存在于成纤维细胞中,成纤维细胞在心脏纤维化中起着关键作用。此外,我们发现司美格鲁肽降低了心脏胶原含量并减轻了肥胖引起的心室壁肥厚。因此,我们的研究结果表明,成纤维细胞表达的 Serpinh1 和 Pcolce 可能在肥胖性心脏纤维化的发展中起作用。通过降低 Serpinh1 和 Pcolce 的表达并延缓心脏纤维化,司美格鲁肽可能具有心脏保护作用。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验