Ahmed Waseem, Azmat Rafia, Chendouh-Brahmi Nabila, Ahmed Rasheed, Naz Saima, Qayyum Abdul, Askary Ahmad El, Gharib Amal F, Alrehaili Amani A, Ali Nausad
Department of Horticulture, The University of Haripur, Pakistan.
Department of Chemistry, University of Karachi, Pakistan.
Saudi J Biol Sci. 2022 Aug;29(8):103351. doi: 10.1016/j.sjbs.2022.103351. Epub 2022 Jul 1.
Antibacterial drug-resistant strains are a serious problem of bacterial treatments nowadays and have a concern. The plant exacts of and are well-known for their role as antibiotic agents. The extraction of novel antibiotic compounds was done by HPLC-DAD, their yield is quantified by numerous solvents. The complete biological activity with antioxidants, bio-kinematicof four compounds of B-Sitosteryl linoleate, Myristyl diglucoside, D-Triglucopyranoside, and S- allylcysteine acids were studied. The supercritical fluid extraction techniques were the best strategies for higher yield, accuracy clarity, and inter, intra process of all four compounds. and samples and investigated in six different solvents. D-Triglucopyranoside (13.81 ± 0.48%), Myristyl diglucoside (11.81 ± 0.41%), B- Sitosteryl linoleate (12.81 ± 0.48%), and s-allylcysteine acids (14.81 ± 0.31%) were higher. The design and action of compounds were applied to proper compartmental pharmacokinetic modelling for in-depth design understanding. The morphology and structure of bacterial cells with the extracted compounds upheld the permeability of cell membranes, membrane integrity, and membrane potential and lower the bacterial binding capacity the infectious index was measured in transmission electron microscopy (TEM) and their alteration process. Plants have well upheld the cellular permeability The toxicity test was performed on both extracted samples with concentrations (1, 0.4, and 0.8%). The areas under plasma half-life of compounds with their solubility, abortion level were higher in four compounds showed the potential of novel antibiotics. The novel medicinal plants used as antibiotics could be the best sources of infection control as a source of future medicines with antibacterial potential solving multidrug issues of bacteria in the world.
抗菌耐药菌株是当今细菌治疗中的一个严重问题，令人担忧。植物提取物和以其作为抗生素的作用而闻名。通过高效液相色谱-二极管阵列检测法（HPLC-DAD）提取新型抗生素化合物，并用多种溶剂对其产量进行定量。研究了四种化合物——β-谷甾醇亚油酸酯、肉豆蔻基二葡糖苷、D-三葡萄糖苷和S-烯丙基半胱氨酸的抗氧化、生物动力学等完整生物活性。超临界流体萃取技术是提高这四种化合物产量、准确性、清晰度以及萃取过程中批内和批间重复性的最佳策略。对和样品在六种不同溶剂中进行了研究。D-三葡萄糖苷（13.81±0.48%）、肉豆蔻基二葡糖苷（11.81±0.41%）、β-谷甾醇亚油酸酯（12.81±0.48%）和S-烯丙基半胱氨酸（14.81±0.31%）的含量较高。将化合物的设计和作用应用于适当的房室药代动力学模型，以深入理解设计。用提取的化合物处理细菌细胞的形态和结构维持了细胞膜的通透性、膜完整性和膜电位，并降低了细菌结合能力，在透射电子显微镜（TEM）中测量了感染指数及其变化过程。植物很好地维持了细胞通透性。对两种提取物样品进行了浓度为（1%、0.4%和0.8%）的毒性试验。四种化合物中，化合物的血浆半衰期与其溶解度、清除水平相关的曲线下面积较高，显示出新型抗生素的潜力。用作抗生素的新型药用植物可能是控制感染的最佳来源，作为未来具有抗菌潜力的药物来源，可解决全球细菌的多重耐药问题。
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