Department of Bioengineering and Therapeutic Sciences, University of California San Francisco, San Francisco, California, USA.
Institute for Human Genetics, University of California San Francisco, San Francisco, California, USA.
Genome Biol. 2022 Jul 18;23(1):159. doi: 10.1186/s13059-022-02727-6.
The most stable structure of DNA is the canonical right-handed double helix termed B DNA. However, certain environments and sequence motifs favor alternative conformations, termed non-canonical secondary structures. The roles of DNA and RNA secondary structures in transcriptional regulation remain incompletely understood. However, advances in high-throughput assays have enabled genome wide characterization of some secondary structures. Here, we describe their regulatory functions in promoters and 3'UTRs, providing insights into key mechanisms through which they regulate gene expression. We discuss their implication in human disease, and how advances in molecular technologies and emerging high-throughput experimental methods could provide additional insights.
DNA 最稳定的结构是被称为 B DNA 的典型右手双螺旋。然而，某些环境和序列基序有利于其他构象，称为非典型二级结构。DNA 和 RNA 二级结构在转录调控中的作用仍不完全清楚。然而，高通量测定方法的进步使人们能够对某些二级结构进行全基因组特征描述。在这里，我们描述了它们在启动子和 3'UTR 中的调节功能，深入了解了它们调节基因表达的关键机制。我们讨论了它们在人类疾病中的意义，以及分子技术的进步和新兴的高通量实验方法如何提供更多的见解。
Zotero 插件
