Department of Pediatric and Preventive Dentistry, JSS Dental College and Hospital, JSS Academy of Higher Education and Research, Mysore, Karnataka, India.
Department of Public Health Dentistry, JSS Dental College and Hospital, JSS Academy of Higher Education and Research, Mysore, Karnataka, India.
J Indian Soc Pedod Prev Dent. 2022 Apr-Jun;40(2):195-200. doi: 10.4103/jisppd.jisppd_193_21.
A number of media that create the best possible conditions to maintain periodontal ligament (PDL) cell viability after dental avulsion have been reported.
The aim of this study is to evaluate ice apple water (IAW), Aloe vera, and propolis as a storage medium to preserve the viability of human PDL fibroblasts.
An in vitro comparative type of study was performed on a PDL cell culture model. PDL fibroblasts obtained from the roots of healthy premolars were cultured in Dulbecco's Modified Eagle's Medium (DMEM) and treated with ice apple water (IAW), 7% propolis extract (PE), 30% Aloe vera extract (AVE), positive control DMEM supplemented with fetal bovine serum, negative control (NC) without any agent, and incubated at 37°C for 1 h, 3 h, and 24 h. Cell viability was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay after every test period. Optical density was measured at a wavelength of 490 nm.
The effects of the test storage media were evaluated by one-way analysis of variance test, followed by post hoc Tukey's multiple comparison test (P < 0.05).
Seven percent PE demonstrated the highest capacity of maintaining PDL cell viability at 1 h and 24 h. IAW showed a statistically significantly lower percentage of viable cells at all three test periods as compared to 7% PE. After 3 h, 30% AVE demonstrated maximum viable cells.
Within the limitations of this study, propolis at a concentration of 7% was the most effective medium for maintaining PDL cell viability.
有许多媒体报道了为保持牙周韧带（PDL）细胞活力而创造的最佳条件。
本研究旨在评估冰苹果水（IAW）、芦荟和蜂胶作为储存介质来保存人牙周膜成纤维细胞活力的效果。
在体外比较研究中，我们使用了牙周膜成纤维细胞培养模型。从健康前磨牙的根部分离获得牙周膜成纤维细胞，在 DMEM 中培养，并分别用冰苹果水（IAW）、7%蜂胶提取物（PE）、30%芦荟提取物（AVE）、含胎牛血清的阳性对照 DMEM、无任何药物的阴性对照（NC）处理，在 37°C 下孵育 1 h、3 h 和 24 h。每个测试期后，使用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）比色法评估细胞活力。在 490nm 波长下测量吸光度。
使用单因素方差分析评估测试储存介质的效果，然后进行事后 Tukey 多重比较检验（P<0.05）。
7% PE 在 1 h 和 24 h 时保持 PDL 细胞活力的能力最高。IAW 在所有三个测试期的活细胞百分比均明显低于 7% PE。3 h 后，30% AVE 显示出最高的活细胞数。
在本研究的限制范围内，浓度为 7%的蜂胶是保持 PDL 细胞活力的最有效介质。
