College of Veterinary Medicine, Zhengzhou, People's Republic of China.
Institute of Microbiology and Epizootics, Centre for Infection Medicine, Department of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany.
Microbiol Spectr. 2022 Aug 31;10(4):e0015422. doi: 10.1128/spectrum.00154-22. Epub 2022 Jul 6.
The horizontal transfer of genomic islands is essential for the adaptation and evolution of Enterococcus faecalis. In this study, three porcine E. faecalis strains, each harboring a large (E)-carrying genomic island, were identified. When using the E. faecalis OG1RF as the recipient, the horizontal transfer of the (E)-carrying genomic island occurred only from E. faecalis E512, which also harbored a pheromone-responsive conjugative plasmid, but not from the other two E. faecalis strains, E533 and E509, which lacked such a plasmid. Subsequently, through plasmid curing of E. faecalis E512 and plasmid introduction into E. faecalis E533, the pheromone-responsive conjugative plasmid was identified to be indispensable for the horizontal transfer of the (E)-carrying genomic island and a subsequent homologous recombination between the chromosomal DNA of the donor and the recipient. In addition, the presence of a chromosomally-located conjugative transposon, Tn, in E. faecalis E509 could not mediate the horizontal transfer of the (E)-carrying genomic island, although Tn itself could transfer by conjugation. Thus, these data highlight the role of the pheromone-responsive conjugative plasmid in the transfer of the (E)-carrying genomic island in E. faecalis, thereby establishing the dual role of pheromone-responsive conjugative plasmids in contributing to the dissemination of both plasmid-borne resistance genes and chromosomally-located genomic islands. In this study, it was shown that a pheromone-responsive conjugative plasmid played an indispensable role in the horizontal transfer of a (E)-carrying genomic island. This finding indicates a dual role of the pheromone-responsive conjugative plasmid in disseminating both plasmid-borne resistance genes and chromosomally-located genomic islands. The role of the pheromone-responsive conjugative plasmid in disseminating chromosomal genomic islands is suggested to be essential in the genomic evolution of E. faecalis, which has become one of the leading nosocomial pathogens worldwide.
基因组岛的水平转移对于粪肠球菌的适应和进化至关重要。在本研究中，鉴定了三株携带大型（E）携带基因组岛的猪源粪肠球菌。当使用粪肠球菌 OG1RF 作为受体时，（E）携带基因组岛的水平转移仅发生在携带与性诱剂反应的可接合质粒的粪肠球菌 E512 中，而不是在其他两株缺乏这种质粒的粪肠球菌 E533 和 E509 中。随后，通过粪肠球菌 E512 的质粒消除和质粒导入粪肠球菌 E533，鉴定出与性诱剂反应的可接合质粒对于（E）携带基因组岛的水平转移以及供体和受体染色体 DNA 之间的同源重组是不可或缺的。此外，尽管可接合转座子 Tn 存在于粪肠球菌 E509 的染色体上，但不能介导（E）携带基因组岛的水平转移，尽管 Tn 本身可以通过接合转移。因此，这些数据突出了与性诱剂反应的可接合质粒在粪肠球菌中（E）携带基因组岛转移中的作用，从而确立了与性诱剂反应的可接合质粒在传播质粒携带的抗性基因和染色体定位的基因组岛方面的双重作用。在本研究中，表明与性诱剂反应的可接合质粒在（E）携带基因组岛的水平转移中发挥了不可或缺的作用。这一发现表明与性诱剂反应的可接合质粒在传播质粒携带的抗性基因和染色体定位的基因组岛方面具有双重作用。性诱剂反应的可接合质粒在传播染色体基因组岛方面的作用对于粪肠球菌的基因组进化至关重要，粪肠球菌已成为全球主要的医院病原体之一。
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