Department of Chemistry, National Institute of Technology, Rourkela, 769008, India.
Department of Chemistry, National Institute of Technology, Rourkela, 769008, India.
Anal Biochem. 2022 Oct 1;654:114813. doi: 10.1016/j.ab.2022.114813. Epub 2022 Jul 19.
Carbon dots have been prepared from resorcinol and sucrose (rsCDs) hydrothermally, which show green emission at 525 nm with a fluorescence quantum yield (PLQY) of 17.2%. The intense emission of rsCDs is quenched upon the addition of Cu. In the presence of bilirubin (BR), the emission intensity is enhanced due to the competitive binding of Cu with bilirubin and hence releasing rsCDs to the sensing medium. It is the first time report on turn-on fluorescence sensing towards BR with a detection limit of 85 nM. Even in the presence of other comparable biomolecules, the sensor is selective and ultrasensitive to bilirubin. A cellulose paper-based sensor strip has also been designed for the naked-eye detection of BR in blood serum. Due to the specific recognition of this rsCDs towards BR, it can be applied to detect BR in practical human serum samples.
碳点是用水热法从间苯二酚和蔗糖（rsCDs）制备的，在 525nm 处显示出绿色发射，荧光量子产率（PLQY）为 17.2%。rsCDs 的强烈发射在加入 Cu 后被猝灭。在胆红素（BR）存在下，由于 Cu 与胆红素的竞争结合，从而将 rsCDs 释放到感测介质中，发射强度增强。这是首次报道对 BR 进行开启荧光传感，检测限为 85nM。即使在存在其他类似生物分子的情况下，该传感器对胆红素也具有选择性和超灵敏性。还设计了一种基于纤维素纸的传感器条，用于在血清中进行 BR 的肉眼检测。由于 rsCDs 对 BR 的特异性识别，它可用于检测实际人血清样本中的 BR。
ACS Appl Mater Interfaces. 2020-7-22
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