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洪水诱导的根际梭菌群落通过促进根质外屏障的形成来阻止水稻根系向地上部转运镉。

Flooding-induced rhizosphere Clostridium assemblage prevents root-to-shoot cadmium translocation in rice by promoting the formation of root apoplastic barriers.

机构信息

Key Lab of Bio-Organic Fertilizer Creation, Ministry of Agriculture and Rural Affairs, Anhui Science and Technology University, Bengbu 233100, China.

School of Medicine, Zhejiang University, Hangzhou 310058, China; School of Life Science and Technology, Tongji University, Shanghai 200092, China.

出版信息

J Hazard Mater. 2022 Oct 5;439:129619. doi: 10.1016/j.jhazmat.2022.129619. Epub 2022 Jul 16.

Abstract

Water managements are the most effective agricultural practices for restraining cadmium (Cd) uptake and translocation in rice, which closely correlated with rhizosphere assembly of beneficial microbiome. However, the role of the assemblage of specific microbiota in controlling root-to-shoot Cd translocation in rice remains scarcely clear. The aim of this study was to ascertain how water managements shaped rhizosphere microbiome and mediated root-to-shoot Cd translocation. To disentangle the acting mechanisms of water managements, we performed an experiment monitoring Cd uptake and transport in rice and changes in soil microbial communities in response to continuously flooding and moistening irrigation. Continuously flooding changed rhizosphere microbial communities, leading to the increased abundance of anaerobic bacteria such as Clostridium populations. Weighted gene co-expression network analysis (WGCNA) showed that a dominant OTU163, corresponding to Clostridium sp. CSP1, exhibited a strong negative correlation with root-to-shoot Cd translocation. An integrated analysis of transcriptome and metabolome further indicated that the Clostridium-secreted butyric acid was involved in the regulation of phenylpropanoid pathway in rice roots. The formation of endodermal suberized barriers and lignified xylems was remarkably enhanced in the Clostridium-treated roots, which led to more Cd retained in root cell wall and less Cd in the xylem sap. Collectively, our results indicate that the development of root apoplastic barriers can be orchestrated by beneficial Clostridium strains that are assembled by host plants grown under flooding regime, thereby inhibiting root-to-shoot Cd translocation.

摘要

水管理是抑制水稻吸收和转运镉(Cd)的最有效农业措施,这与根际有益微生物组的组装密切相关。然而,特定微生物群落的组合在控制水稻根部到茎部 Cd 转运中的作用仍不清楚。本研究旨在确定水管理如何塑造根际微生物组并介导根部到茎部 Cd 转运。为了厘清水管理的作用机制,我们进行了一项实验,监测水稻对 Cd 的吸收和转运以及土壤微生物群落对持续淹水和湿润灌溉的响应变化。持续淹水改变了根际微生物群落,导致厌氧细菌(如梭菌属)的丰度增加。加权基因共表达网络分析(WGCNA)表明,一个主要的 OTU163,对应于梭菌属 CSP1,与根部到茎部 Cd 转运呈强烈负相关。转录组和代谢组的综合分析进一步表明,梭菌分泌的丁酸参与了水稻根部苯丙烷途径的调控。在梭菌处理的根中,内皮层的木质素栓化屏障和木质部的木质化明显增强,导致更多的 Cd 保留在根细胞壁中,而木质部汁液中的 Cd 减少。总之,我们的结果表明,有益的梭菌菌株可以通过在淹水条件下生长的宿主植物来组装,从而形成根质外体屏障,抑制根部到茎部 Cd 转运。

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