School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Areas, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, 800 Dongchuan Rd, Shanghai 200240, China.
School of Agriculture and Biology, Shanghai Jiao Tong University, 200240, China; Key Laboratory of Urban Agriculture, Ministry of Agriculture and Rural Areas, Shanghai Jiao Tong University, Shanghai 200240, China; Shanghai Yangtze River Delta Eco-Environmental Change and Management Observation and Research Station, Ministry of Science and Technology, Ministry of Education, 800 Dongchuan Rd, Shanghai 200240, China; Yunnan Dali Research Institute, Shanghai Jiao Tong University, Dali, Yunnan 671000, China.
J Hazard Mater. 2022 Oct 5;439:129640. doi: 10.1016/j.jhazmat.2022.129640. Epub 2022 Jul 20.
Carbon nanotubes present potential applications in soil remediation, particularly in phytoremediation. Yet, how multi-walled carbon nanotubes (MWCNTs) induced hyperaccumulator growth at molecular level remains unclear. Here, physio-biochemical, transcriptomic, and metabolomic analyses were performed to determine the effect of MWCNTs on Solanum nigrum L. (S. nigrum) growth under cadmium and arsenic stresses. 500 mg/kg MWCNTs application significantly promoted S. nigrum growth, especially for root tissues. Specially, MWCNTs application yields 1.38-fold, 1.56-fold, and 1.37-fold enhancement in the shoot length, root length, and fresh biomass, respectively. Furthermore, MWCNTs significantly strengthened P and Fe absorption in roots, as well as the activities of antioxidative enzymes. Importantly, the transcriptomic analysis indicated that S. nigrum gene expression was sensitive to MWCNTs, and MWCNTs upregulated advantageous biological processes under heavy metal(loid)s stress. Besides, MWCNTs reprogramed metabolism that related to defense system, leading to accumulation of 4-hydroxyphenylpyruvic acid (amino acid), 4-hydroxycinnamic acid (xenobiotic), and (S)-abscisic acid (lipid). In addition, key common pathways of differentially expressed metabolites and genes, including "tyrosine metabolism" and "isoquinoline alkaloid biosynthesis" were selected via integrating transcriptome and metabolome analyses. Combined omics technologies, our findings provide molecular mechanisms of MWCNTs in promoting S. nigrum growth, and highlight potential application of MWCNTs in soil remediation.
碳纳米管在土壤修复中具有潜在的应用，特别是在植物修复中。然而，多壁碳纳米管（MWCNTs）如何在分子水平上诱导超积累植物的生长仍不清楚。在这里，进行了生理生化、转录组和代谢组分析，以确定 MWCNTs 在镉和砷胁迫下对龙葵（S. nigrum）生长的影响。500mg/kg MWCNTs 的应用显著促进了龙葵的生长，特别是根组织。特别是，MWCNTs 的应用分别使地上部分长度、根长和鲜生物量增加了 1.38 倍、1.56 倍和 1.37 倍。此外，MWCNTs 显著增强了根部对 P 和 Fe 的吸收，以及抗氧化酶的活性。重要的是，转录组分析表明，MWCNTs 对龙葵基因表达敏感，MWCNTs 在重金属（类）胁迫下上调了有利的生物学过程。此外，MWCNTs 重新编程了与防御系统相关的代谢，导致 4-羟基苯丙酮酸（氨基酸）、4-羟基肉桂酸（异源生物）和（S）-脱落酸（脂质）的积累。此外，通过整合转录组和代谢组分析，选择了差异表达代谢物和基因的关键共同途径，包括“酪氨酸代谢”和“异喹啉生物碱生物合成”。综合组学技术，我们的研究结果提供了 MWCNTs 促进龙葵生长的分子机制，并强调了 MWCNTs 在土壤修复中的潜在应用。
