Gómez Rocío, Neri-Bazán Rocío M, Posadas-Mondragon Araceli, Vizcaíno-Dorado Pablo A, Magaña Jonathan J, Aguilar-Faisal José Leopoldo
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados del IPN (CINVESTAV-IPN), Mexico City 07360, Mexico.
Laboratorio de Medicina de Conservación, Escuela Superior de Medicina-Instituto Politécnico Nacional (ESM-IPN), Mexico City 11340, Mexico.
Animals (Basel). 2022 Jul 21;12(14):1857. doi: 10.3390/ani12141857.
Genetic analysis is a conventional way of identifying and monitoring captive and wildlife species. Knowledge of statistical parameters reinforcing their usefulness and effectiveness as powerful tools for preserving diversity is crucial. Although several studies have reported the diversity of cetaceans such as using microsatellites, its informative degree has been poorly reported. Furthermore, the genetic structure of this cetacean has not been fully studied. In the present study, we selected 15 microsatellites with which 210 dolphins were genetically characterized using capillary electrophoresis. The genetic assertiveness of this set of hypervariable markers identified one individual in the range of 6.927e to 1.806e, demonstrating its substantial capability in kinship relationships. The genetic structure of these 210 dolphins was also determined regarding the putative capture origin; a genetic stratification ( = 2) was found. An additional dolphin group of undetermined origin was also characterized to challenge the proficiency of our chosen markers. The set of markers proposed herein could be a helpful tool to guarantee the maintenance of the genetic diversity rates in conservation programs both in and across other odontocetes, Mysticeti and several genera of endangered and vulnerable species.
遗传分析是识别和监测圈养及野生生物物种的传统方法。了解强化其作为保护生物多样性有力工具的有用性和有效性的统计参数至关重要。尽管有几项研究报告了鲸类动物的多样性,例如使用微卫星,但对其信息丰富程度的报道却很少。此外,这种鲸类动物的遗传结构尚未得到充分研究。在本研究中,我们选择了15个微卫星,利用毛细管电泳对210只海豚进行了基因特征分析。这组高变标记的基因确定性在6.927e至1.806e范围内识别出一个个体,证明了其在亲缘关系方面的强大能力。还根据假定的捕获来源确定了这210只海豚的遗传结构;发现了一个遗传分层(=2)。另外还对一组来源不明的海豚进行了特征分析,以检验我们所选标记的有效性。本文提出的这组标记可能是一种有用的工具,可确保在保护计划中维持齿鲸、须鲸以及其他几类濒危和易危物种内部及之间的遗传多样性比率。