Perussolo Maiara Carolina, Mogharbel Bassam Felipe, Saçaki Claudia Sayuri, Dziedzic Dilcele Silva Moreira, Nagashima Seigo, de Meira Leanderson Franco, Guarita-Souza Luiz Cesar, de Noronha Lúcia, Athayde Teixeira de Carvalho Katherine
Advanced Therapy and Cellular Biotechnology in Regenerative Medicine Department, The Pelé Pequeno Príncipe Research Institute, Child and Adolescent Health Research & Pequeno Príncipe Faculties, Curitiba 80240-020, PR, Brazil.
Laboratory of Experimental Pathology, Graduate Program of Health Sciences, School of Medicine, Paraná Pontifical Catholic University (PUCPR), Curitiba 80215-901, PR, Brazil.
Life (Basel). 2022 Jun 27;12(7):962. doi: 10.3390/life12070962.
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system, characterized as an inflammatory demyelinating disease. Given the need for improvements in MS treatment, many studies are mainly conducted through preclinical models such as experimental allergic encephalomyelitis (EAE). This study analyzes the relationships between histopathological and clinical score findings at EAE. Twenty-three female Lewis rats from 6 to 8 weeks were induced to EAE. Nineteen rats underwent EAE induction distributed in six groups to establish the evolution of clinical signs, and four animals were in the control group. toxin (PTX) doses were 200, 250, 300, 350 and 400 ng. The clinical scores of the animals were analyzed daily, from seven to 24 days after induction. The brains and spinal cords were collected for histopathological analyses. The results demonstrated that the dose of 250 ng of PTX induced a higher clinical score and reduction in weight. All induced groups demonstrated leukocyte infiltration, activation of microglia and astrocytes, and demyelinated plaques in the brains in histopathology. It was concluded that the dose of 250 ng and 350 ng of PTX were the best choices to trigger the brain and spinal cord demyelination lesions and did not correlate with clinical scores.
多发性硬化症（MS）是一种中枢神经系统的自身免疫性疾病，其特征为炎性脱髓鞘疾病。鉴于多发性硬化症治疗需要改进，许多研究主要通过实验性变应性脑脊髓炎（EAE）等临床前模型进行。本研究分析了EAE中组织病理学和临床评分结果之间的关系。选用23只6至8周龄的雌性Lewis大鼠诱导其发生EAE。19只大鼠接受EAE诱导并分为六组以确定临床症状的演变，4只动物作为对照组。百日咳毒素（PTX）剂量分别为200、250、300、350和400纳克。诱导后7至24天每天分析动物的临床评分。收集大脑和脊髓进行组织病理学分析。结果表明，250纳克的PTX剂量诱导出更高的临床评分和体重减轻。所有诱导组在组织病理学上均显示大脑中有白细胞浸润、小胶质细胞和星形胶质细胞活化以及脱髓鞘斑块。得出的结论是，250纳克和350纳克的PTX剂量是引发脑和脊髓脱髓鞘病变的最佳选择，且与临床评分无关。
